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Cryopreservation of shoot tips of endangered Hayachine-usuyukiso (Leontopodium hayachinense (Takeda) Hara et Kitam.) using a vitrification protocol
https://iwate-u.repo.nii.ac.jp/records/9150
https://iwate-u.repo.nii.ac.jp/records/9150c053afff-292c-4467-8037-daacc4489082
名前 / ファイル | ライセンス | アクション |
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pgrcu-v6n2p164-166.pdf (496.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-01-13 | |||||
タイトル | ||||||
タイトル | Cryopreservation of shoot tips of endangered Hayachine-usuyukiso (Leontopodium hayachinense (Takeda) Hara et Kitam.) using a vitrification protocol | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cryopreservation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | endangered species | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Leontopodium hayachinense (Takeda) Hara et Kitam | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | tissue culture; vitrification | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Tanaka, Daisuke
× Tanaka, Daisuke× Niino, Takao× Tsuchiya, Yoshiko× Shirata, Kazuto× Uemura, Matsuo |
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著者(機関) | ||||||
Genebank, National Institute of Agrobiological Sciences | ||||||
著者(機関) | ||||||
Genebank, National Institute of Agrobiological Sciences | ||||||
著者(機関) | ||||||
Graduate School of Agriculture, Iwate University | ||||||
著者(機関) | ||||||
Genebank, National Institute of Agrobiological Sciences | ||||||
著者(機関) | ||||||
Graduate School of Agriculture, Iwate University | ||||||
登録日 | ||||||
日付 | 2010-01-13 | |||||
書誌情報 |
Plant Genetic Resources: Characterization and Utilization 巻 6, 号 2, p. 164-166, 発行日 2008-01-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1479-2621 | |||||
Abstract | ||||||
内容記述タイプ | Other | |||||
内容記述 | Hayachine-usuyukiso (Leontopodium hayachinense) is an alpine plant native to Mt Hayachine. This unique chrysanthemum is listed as an endangered plant by the Department of Conservation, Iwate Prefecture, and as a threatened plant by the Ministry of the Environment, Japan. We successfully cryopreserved the shoot tips from in vitro-grown L. hayachinense shoots using a vitrification protocol. Cold-hardened shoot tips were excised and pre-cultured on a solidified Murashige–Skoog medium containing 0.3M sucrose for 1 d at 58C. The shoot tips were then treated with loading solution for 20 min at 258C, dehydrated in plant vitrification solution 2 for 120 min at 258C and immersed in liquid nitrogen. The survival rate of the vitrified shoot tips was 63.3% after 30 d of regrowth. This protocol appears to be a promising technique for the cryopreservation of in vitro-grown shoots of this endangered plant. |
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出版者 | ||||||
出版者 | Cambridge University Press | |||||
権利 | ||||||
権利情報 | Copyright © NIAB 2008 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1017/S1479262108993175 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |