We studied the influence of fuel and lubricant oil on the combustion chamber deposit (CCD) formation using an autoclave and a small two-stroke engine. The autoclave results showed that there were some temperature and pressure region that made a maximum deposit formation. On the other hand, the CCD weight on the piston crown surface of a utility two-stroke cycle spark ignition engine was measured, and the relative contribution of fuel and lubricant oil to the CCD formation was discussed. The engine was operated with gasoline as a fuel and with commercial oil for two-stroke and base oil without any additives as lubricant oil. As a result, most CCD was formed from lubricant oils, especially oil additives. Furthermore, we measured the temperature profiles on a piston crown surface using many fusible plugs that had specific melting points, for considering the relation between the engine CCD and the autoclave results.