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Root sampling and RNA extraction methods for field-based gene expression analysis of soybeans
https://iwate-u.repo.nii.ac.jp/records/15502
https://iwate-u.repo.nii.ac.jp/records/15502c610fe53-3825-4499-a72c-ec4d40611636
名前 / ファイル | ライセンス | アクション |
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pps-v24n3p339-345 (3.0 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-01-05 | |||||
タイトル | ||||||
タイトル | Root sampling and RNA extraction methods for field-based gene expression analysis of soybeans | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Aquaporin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | RNA extraction | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | root | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | soybean | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
OKAMURA, Shin
× OKAMURA, Shin× Mumtahina, Nabila× SHIMONO, Hiroyuki× MATSUNAMI, Maya |
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著者(機関) | ||||||
Department of Plant Bio Sciences, Faculty of Agriculture, Iwate University | ||||||
著者(機関) | ||||||
Graduate School of Arts and Sciences, Division of Agriculture, Iwate University | ||||||
著者(機関) | ||||||
Department of Plant Bio Sciences, Faculty of Agriculture, Iwate University, Agri-Innovation Center, Iwate University | ||||||
著者(機関) | ||||||
Department of Plant Bio Sciences, Faculty of Agriculture, Iwate University | ||||||
登録日 | ||||||
日付 | 2022-01-05 | |||||
書誌情報 |
Plant Production Science 巻 24, 号 3, p. 339-345, 発行日 2020-10-21 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1343943X | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13491008 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Isolation of an adequate quantity of high-quality RNA is a crucial step in gene expression analysis. Difficulties in sampling and RNA extraction of field-grown crops, especially from roots, are substantial obstacles to understanding actual gene expression in the field. We examined the effectiveness of the RNA extraction method (FRP) using pot- and field-grown soybean roots. High quantity and quality of total RNA could extract by the FRP method from both pot- and field-grown soybean roots at different growth stages. To determine the influence of root washing during the sampling process, roots were washed with water for 5 s, 1 min, and 5 min, then analyzed for aquaporin gene expression levels. Root washing for 5 min affected gene expression of some aquaporins. To obtain RNA that reflects the correct gene expression profile, it is necessary to minimize the effects of the sampling process. | |||||
出版者 | ||||||
出版者 | Taylor & Francis Group | |||||
関係URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.tandfonline.com/doi/full/10.1080/1343943X.2020.1834867 | |||||
関連名称 | Root sampling and RNA extraction methods for field-based gene expression analysis of soybeans | |||||
権利 | ||||||
権利情報 | © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. | |||||
権利URI | ||||||
権利情報 | http://creativecommons.org/licenses/by/4.0/ | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1080/1343943X.2020.1834867 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |