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Specific Inheritance of a Mutant Gene Controlling α,β Subunits-Null of β-Conglycinin in Soybean (Glycine max (L.) Merrill) and Observation of Chloroplast Ultrastructure of the Mutant
https://iwate-u.repo.nii.ac.jp/records/9323
https://iwate-u.repo.nii.ac.jp/records/9323e23f2608-1bcd-40f2-8dbf-54c6ee014cce
名前 / ファイル | ライセンス | アクション |
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bs-v46n1p53-59.pdf (836.7 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2012-01-13 | |||||
タイトル | ||||||
タイトル | Specific Inheritance of a Mutant Gene Controlling α,β Subunits-Null of β-Conglycinin in Soybean (Glycine max (L.) Merrill) and Observation of Chloroplast Ultrastructure of the Mutant | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Soybean (Glycine max ) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | seed protein | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | y-ray irradiation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | protein subunit null mutant | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | lethal chlorosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | distorted segregation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | chloroplast ultrastructure. | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
PHAN, Ton Huu
× PHAN, Ton Huu× KAIZUMA, Norihiko× ODANAKA, Hiroya× TAKAHATA, Yoshihito |
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著者(機関) | ||||||
Faculty of Agriculture, Iwate University | ||||||
登録日 | ||||||
日付 | 2012-01-13 | |||||
書誌情報 |
Breeding science 巻 46, 号 1, p. 53-59, 発行日 1996-03-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1344-7610 | |||||
Abstract | ||||||
内容記述タイプ | Other | |||||
内容記述 | ダイズ種子貯蔵タンパク質の栄養価の改善をβ一コンクリシニンの生成量の減少又はグリシニン生成量の増加によって実現するため,ワセスズナリ品種約3,000粒の乾燥種子に対し1Oから50kRのγ線照射を行った.3,500粒のM_2種子を得て,それらからSDS-PAGEによりタンパク質構成に関する突然変異体の検出を試みた.その結果,β-コンクリシニンのα及びβサブユニット双方がともに欠失している突然変異体が得られたが,それは同時に幼苗期に致死的な葉緑変異を引き起こすものであった. その突然変異形質の分離様式がM3からM8世代にかけて研究され,α及びβサブユニットタンパク質の生成量に関して,3正常:4半畳:1欠失という異常分離比がその前の世代のヘテロ遺伝子型個体の着生種子から明かにされた.相反交雑実験が突然変異形質に関するヘテロ遺伝子型と正常形質ホモ遺伝子型の毛根品種との間で行われ,その結果,この突然変異形質はある劣性突然変異遺伝子(α)で支配されていること,その劣性遺伝子(α)を持つ花粉は正常遺伝子(λ)を持つ花粉よりも少なく生成され,その比がα:A=1:3であると結論づけられた.さらに欠失変異体(aa)の葉緑体の超微的構造が電子顕微鏡的に観察された. | |||||
Abstract | ||||||
内容記述タイプ | Other | |||||
内容記述 | In order to improve the nutritional value of soybean protein through the decrease of the β-conglycinin content or increase of the glycinin content, 3,000 dry seeds of a culti-var 'Wasesuzunari' were treated with 10 to 50 kR y -rays. The 3,500 M2 seeds were observed for the analysis of the protein composition of the mutants by SDS-PAGE. As a result, a mutant trait characterized by the lack of both the α and β subunits of β-conglycinin and lethal chiorosis was detected. Investigation on the segregation of the mutant trait was carried out from the M3 to M8 generations and a peculiar segregation ratio (3 normal : 4 with half the amount : I null for the α,β subunits) was obtained from heterozygous individuals from the previous generations. | |||||
出版者 | ||||||
出版者 | 日本育種学会 | |||||
権利 | ||||||
権利情報 | 日本育種学会 | |||||
その他のタイトル | ||||||
その他のタイトル | ダイズ(Glycthe max (L.) Merrill) におけるβ-コンクリシニンのα,βサブユニット欠失突然変異の特殊な遺伝様式及び突然変異体における葉緑体の超微細構造の観察 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |