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KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media
https://iwate-u.repo.nii.ac.jp/records/15507
https://iwate-u.repo.nii.ac.jp/records/155072805a985-e2ca-4780-b12a-e1f92b0f23b1
名前 / ファイル | ライセンス | アクション |
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jps-v58n4p270-279 (3.0 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-01-07 | |||||
タイトル | ||||||
タイトル | KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cell culture | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cell growth | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cellular senescence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | chick fibroblasts | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA damage | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | growth medium | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
KATAYAMA, Masafumi
× KATAYAMA, Masafumi× ONUMA, Manabu× FUKUDA, Tomokazu |
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著者(機関) | ||||||
Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, Wildlife Genome Collaborative Research Group, National Institute for Environmental Studies | ||||||
著者(機関) | ||||||
Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, Wildlife Genome Collaborative Research Group, National Institute for Environmental Studies | ||||||
著者(機関) | ||||||
Wildlife Genome Collaborative Research Group, National Institute for Environmental Studies, Graduate School of Science and Engineering | ||||||
登録日 | ||||||
日付 | 2022-01-07 | |||||
書誌情報 |
The journal of poultry science 巻 58, 号 4, p. 270-279, 発行日 2021 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13490486 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13467395 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Cultured cells are a useful resource for poultry scientists, since these cells allow scientists to evaluate biological responses to conditions such as infectious diseases in vitro while mimicking the whole-body response in birds. However avian cell culture requires an optimized basal medium, and there are currently relatively few options for this basal medium (medium 199 and KAv-1). This means that there is still room for the development of an optimal basal medium for avian cell culture. Here we compare KAv-1 medium, Dulbecco's modified Eagle medium (DMEM) and medium 199 during the culture of chick fibroblasts and determine that KAv-1 remains the optimal medium for these assays. Our results show that DNA damage is reduced in fibroblasts cultured in the KAv-1 medium, when compared to both DMEM and Medium 199 and that these cells also display improved growth dynamics in KAv-1 medium when compared to both DMEM and medium 199. To the best of our knowledge, this is the first study to describe a comparative analysis of culture media for avian cells, which would provide useful information for poultry scientists. | |||||
抄録(URL) | ||||||
KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media | ||||||
https://www.jstage.jst.go.jp/article/jpsa/58/4/58_0200085/_article | ||||||
出版者 | ||||||
出版者 | Japan Poultry Science Association | |||||
権利 | ||||||
権利情報 | Ⓒ 2021 Japan Poultry Science Association | |||||
権利URI | ||||||
権利情報 | https://creativecommons.org/licenses/by-nc-sa/4.0/ | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11564513 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12018767 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.2141/jpsa.0200085 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |