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ガラス化法およびビーズガラス化法を用いた培養植物茎頂の超低温保存 Cryopreservation of In Vitro-Grown Plant Apical Shoot Tips by Vitrification and Encapsulation-Vitrification 田中, 大介 新野, 孝男 五十鈴川, 寛司 日影, 孝志 上村, 松生 低温生物工学会 ご利用に当たっては「著作権法」に従うことをお願いいたします． Cryopreservation Cold acclimation Dehydration tolerance Vitrification Encapsulation-Vitrification 超低温保存 低温馴化 脱水耐性 ガラス化法 ビーズガラス化法 In vitro-grown apical shoot tips of Gentiana were successfully cryopreserved by vitrification (V) and encapsulation-vitrification (EV) protocols. We optimized and compared the V and EV protocols. Although both protocols resulted in a relatively high survival rate, the EV protocol seemed to have several distinct advantages. First, the survival rate of shoot tips cryopreserved by the EV protocol was much greater than that by the V protocol under optimized conditions. Second, the range of optimum treatment period by vitrification solution (PVS2 solution) was considerably wider with the EV protocol than with the V protocol. Third, after rewarming from liquid nitrogen temperature, the cryopreserved shoot tips showed more vigorous growth for the EV protocol than the V protocol. With the optimum EV protocol, we succeeded to cryopreserve shoot tips from 10 different lines of Gentiana and uniformly obtained high survival rate. Thus, the EV protocol appears to be promising for cryopreservation of a wide range of plant germplasm. 低温生物工学会 2002-12-28 jpn journal article VoR https://iwate-u.repo.nii.ac.jp/records/9241 1340-7902 低温生物工学会誌 48 2 135 138 https://iwate-u.repo.nii.ac.jp/record/9241/files/cc-v48n2p135-138.pdf application/pdf 191.8 kB 2016-11-14