2026-03-06T01:54:49Z https://iwate-u.repo.nii.ac.jp/oai

oai:iwate-u.repo.nii.ac.jp:00015507 2023-05-15T13:02:32Z 1515:1519

KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media KATAYAMA, Masafumi ONUMA, Manabu FUKUDA, Tomokazu Ⓒ 2021 Japan Poultry Science Association https://creativecommons.org/licenses/by-nc-sa/4.0/ cell culture cell growth cellular senescence chick fibroblasts DNA damage growth medium Cultured cells are a useful resource for poultry scientists, since these cells allow scientists to evaluate biological responses to conditions such as infectious diseases in vitro while mimicking the whole-body response in birds. However avian cell culture requires an optimized basal medium, and there are currently relatively few options for this basal medium (medium 199 and KAv-1). This means that there is still room for the development of an optimal basal medium for avian cell culture. Here we compare KAv-1 medium, Dulbecco's modified Eagle medium (DMEM) and medium 199 during the culture of chick fibroblasts and determine that KAv-1 remains the optimal medium for these assays. Our results show that DNA damage is reduced in fibroblasts cultured in the KAv-1 medium, when compared to both DMEM and Medium 199 and that these cells also display improved growth dynamics in KAv-1 medium when compared to both DMEM and medium 199. To the best of our knowledge, this is the first study to describe a comparative analysis of culture media for avian cells, which would provide useful information for poultry scientists. Japan Poultry Science Association 2021 eng journal article VoR https://iwate-u.repo.nii.ac.jp/records/15507 info:doi/10.2141/jpsa.0200085 AA11564513 AA12018767 13490486 13467395 The journal of poultry science 58 4 270 279 https://iwate-u.repo.nii.ac.jp/record/15507/files/jps-v58n4p270-279.pdf application/pdf 3.0 MB 2022-01-07