2026-03-12T16:12:00Z https://iwate-u.repo.nii.ac.jp/oai

oai:iwate-u.repo.nii.ac.jp:00015755 2023-05-15T12:52:00Z 1504:1510

Molecular detection of filarial nematode parasites in japanese black bears ( Ursus thibetanus japonicus ) from iwate prefecture , japan MASATANI, Tatsunori KOJIMA, Isshu TASHIRO, Michiyo YAMAUCHI, Kiyoshi FUKUI, Daisuke ICHIKAWA-SEK, Madoka HARASAWA, Ryo ©2021 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/ filaria Japanese black bear PCR phylogenetic analysis This study aimed to detect filarial parasites in blood samples of Japanese black bears (Ursus thibetanus japonicus) collected from Iwate Prefecture, Japan. Positive amplicons were obtained from 26 out of 30 samples by nested PCR targeting 18S ribosomal RNA gene and first internal transcribed spacer regions. DNA sequences of Mansonella sp. close to M. ozzardi and Dirofilaria sp. were detected for eight and 11 positive amplicons, respectively. Co-infection was detected for the remaining seven amplicons. Dirofilaria sp. was identified as D. ursi by further genetic analysis of 5S ribosomal RNA gene sequence. The results of this study will contribute to further investigations of Japanese black bears for monitoring their risk as a reservoir of possible zoonotic filarial parasites. Japanese Society of Veterinary Science 2021 eng journal article VoR https://iwate-u.repo.nii.ac.jp/records/15755 info:doi/10.1292/jvms.20-0466 AA10796138 AA11510037 13477439 09167250 Journal of Veterinary Medical Science 83 2 208 213 https://iwate-u.repo.nii.ac.jp/record/15755/files/jvms-v83n2p208-213.pdf application/pdf 2.3 MB 2022-08-03