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        <identifier>oai:iwate-u.repo.nii.ac.jp:00009155</identifier>
        <datestamp>2023-05-16T11:13:08Z</datestamp>
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          <dc:title>ダルベッコ変法イーグル培地を用いたキメラマウス胚の培養成績</dc:title>
          <jpcoar:creator>
            <jpcoar:creatorName>高橋, 正浩</jpcoar:creatorName>
          </jpcoar:creator>
          <jpcoar:creator>
            <jpcoar:creatorName>石原, 由美</jpcoar:creatorName>
          </jpcoar:creator>
          <jpcoar:creator>
            <jpcoar:creatorName>高橋, 寿太郎</jpcoar:creatorName>
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          <jpcoar:creator>
            <jpcoar:creatorName>安田, 泰久</jpcoar:creatorName>
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          <dc:rights>Copyright (c) 1997 日本哺乳動物卵子学会</dc:rights>
          <jpcoar:subject subjectScheme="Other">キメラ胚</jpcoar:subject>
          <jpcoar:subject subjectScheme="Other">DMEM</jpcoar:subject>
          <jpcoar:subject subjectScheme="Other">胚発生</jpcoar:subject>
          <datacite:description descriptionType="Other">培養液としてDMEMを使用して体外培養におけるマウスキメラ胚の発生について検討した．キメラ胚（BALB/c⇔C57BL/6J）の統合胚盤胞以降への発生率は，4細胞期胚同士の集合胚で82.2％，8細胞期胚同士で89.1％，16細胞期胚同士で84.7％となった．8細胞期胚同士の集合胚の発生を経時的に観察すると，統合胚盤胞以降への発生率は培養12時間で0％，24時間で25.4％，36時間で80.4％となり，培養24時間における胚盤胞と培養36時間における拡張胚盤胞への発生率において，単一胚と比較して有意差があり，発生速度の遅延が認められた．</datacite:description>
          <dc:publisher>日本哺乳動物卵子学会</dc:publisher>
          <datacite:date dateType="Issued">1997-01-01</datacite:date>
          <dc:language>jpn</dc:language>
          <dc:type rdf:resource="http://purl.org/coar/resource_type/c_6501">journal article</dc:type>
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          <jpcoar:sourceIdentifier identifierType="ISSN">1341-7738</jpcoar:sourceIdentifier>
          <jpcoar:sourceTitle>日本哺乳動物卵子学会誌</jpcoar:sourceTitle>
          <jpcoar:volume>14</jpcoar:volume>
          <jpcoar:issue>1</jpcoar:issue>
          <jpcoar:pageStart>102</jpcoar:pageStart>
          <jpcoar:pageEnd>105</jpcoar:pageEnd>
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            <datacite:date dateType="Available">2016-11-14</datacite:date>
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