2026-03-07T14:37:13Z https://iwate-u.repo.nii.ac.jp/oai

oai:iwate-u.repo.nii.ac.jp:00015760 2023-05-15T12:51:59Z 1504:1510

Development of conventional multiplex pcr method for discrimination between dispharynx nasuta and cheilospirura hamulosa ( Nematoda : Acuariidae ) parasitizing poultry BISWAS, Peru Gopal OHARI, Yuma MOHANTA, Uday Kumar ITAGAKI, Tadashi ©2021 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/ Cheilospirura hamulosa Dispharynx nasuta multiplex PCR nuclear ribosomal 18S-ITS1-5.8S-ITS2-28S DNA poultry The poultry infections caused by Dispharynx nasuta and Cheilospirura hamulosa nematodes are difficult to be diagnosed by fecal examination because of their egg similarity. In this study, we analyzed DNA sequences of nuclear ribosomal 18S-ITS1-5.8S-ITS2-28S region of D. nasuta and C. hamulosa and developed conventional multiplex PCR method using species-specific primers for discriminating between the two species. The method amplified 455-bp and 319-bp fragments specific to D. nasuta and C. hamulosa, respectively, and did not produce them against the other chicken nematode species, Ascaridia galli, Oxyspirura mansoni, Heterakis gallinarum, Heterakis beramporia, and Heterakis indica, suggesting that the multiplex PCR is sensitive and available for species diagnosis. Japanese Society of Veterinary Science 2021 eng journal article VoR https://iwate-u.repo.nii.ac.jp/records/15760 info:doi/10.1292/jvms.20-0664 AA10796138 AA11510037 13477439 09167250 Journal of Veterinary Medical Science 83 2 226 229 https://iwate-u.repo.nii.ac.jp/record/15760/files/jvms-v83n2p226-229.pdf application/pdf 1.8 MB 2022-08-17