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oai:iwate-u.repo.nii.ac.jp:00009326 2023-05-16T11:05:57Z 1504:1510

Biological Evaluation of Residual Toxicity of Formaldehyde Gas Sterilization and Neutralization Method ホルマリンガス殺菌・無毒化法の残留毒性の生物学的評価 平田, 統一 吉田, 敬 安田, 準 ホルマリンガス 殺菌 無毒化 残留毒性 生物学的評価 Formaldehyde gas sterilization neutralization residual toxicity biological evaluation ホルマリンガス(FG)殺菌・無害化装置「Holl Steri」(アスカメディカル)の残留毒性を、従来法のエチレンオキサイドガス(EOG)殺菌法のそれと比較して、生物学的に評価した。0.25mlストローに6時間保存した精子生存率において、FG区は無殺菌の対照区に比べ6時間目で有意(p＜0.01)に生存率が減少した。しかし、FG区はEOG区に比べて有意(2時問目:p＜0.01、4時間目:p＜0.05)に生存率が高かった。3日間の胚盤胞期牛胚培養において、FG区はガンマ線殺菌の対照区に比べ2日目で有意(p＜0.01)に生存率が減少し、2.5日目にはすべての胚が死滅した。しかし、EOG区に対しては、培養後1、2日目に、有意(p＜0.01)に生存率が高かった。10日間前後の牛卵子の体外成熟・受精・培養系において、FG区およびガンマ線殺菌の対照区の5細胞期以上への分割率と胚盤胞への発生率は、それぞれ60.0%と20.0%および65.0%と36.7%で、FG区でやや低かったものの有意差はなかった。一方、EOG区での胚発生はなかった。以上の結果から、FG殺菌・無毒化装置による殺菌では、無殺菌やガンマ線滅菌に比べ細胞毒性の残存がみられるが、従来広く用いられてきたEOG殺菌に比べ残留毒性は極めて少ないことが示された。 The residual toxicity of formaldehyde gas (FG) sterilization and neutralization equipment, "Holl Steri" (Asuka Medical), was evaluated biologically and compared with the conventional ethylene oxide gas (EOG) disinfectant process. The percentage of FG group motile spermatozoa, preserved in 0.25 ml straws for 6 hours, compared with the non-sterilized control group, decreased significantly (p<0.01) in the 6th hour. However, FG group had significantly higher survival rate than EOG group (2nd hour : p<0.01 ; 4th hour p<0.05). In bovine embryo culture for three days, the embryo survival rate decreased significantly (p<0.01) in FG group compared with control group of gamma ray sterilization on the 2nd day, and, at 2.5 days, all embryos were nonviable. However, compared with EOG group, embryo survival rate in FG group was significantly (p<0.01) higher on the 1 st and 2 nd days. In the in vitro maturation, fertilization and culture system of bovine oocytes for about ten days, the division rates into five or more-celled embryos and the blastocyst generation rates in FG group and control group of gamma ray sterilization were 60.0, and 20.0%, and 65.0 and 36.7%, respectively. Although rates were a little lower in FG group, there was no significant difference between groups. On the other hand, there was no embryonic growth in EOG group. These results suggest there was slight residual cell toxicity with FG sterilization and neutralization equipment compared with non-sterilizing or gamma ray sterilization, however there was very little residual toxicity compared with the widely employed conventional EOG sterilization. journal article 日本家畜臨床学会 2004-11-10 VoR application/pdf 日本家畜臨床学会誌 2 27 51 55 1346-8464 https://iwate-u.repo.nii.ac.jp/record/9326/files/jjvc-v27m2p51-55.pdf https://iwate-u.repo.nii.ac.jp/records/9326 jpn