2026-03-13T02:42:44Z https://iwate-u.repo.nii.ac.jp/oai

oai:iwate-u.repo.nii.ac.jp:00011869 2023-05-15T15:02:15Z 1523:1528:1591:1631:1926

器官培養系での表皮キヤロンの細胞分裂およびDNA合成に対する効果 Separation of cell cycle phase-specific chalones using the epidermal organ culture as an assay system HIROBE, Tomohisa YAMAGUCHI, Takeo assay system epidermal organ culture cell cycle phase-specific chalones When a piece of mouse ear conch was laid on a disk of glass filter immersed in a TC 199 medium and kept at 37°C in an atmosphere of 95 % O2+5 % CO2 gas mixture, an abrupt increase in the number of mitoses and DNA synthesizing cells was observed between 12 and 24 h after the initiation of culture. The skin extract containing epidermal chalone was added to the culture medium at the time on the course of increase in mitoses and DNA synthesizing cells. In the case of DNA synthesizing cells 3H-thymidine was added various times later. Both G2-chalone which inhibited the transition from G2 to M and G1-chalone which inhibited the transition from G1 to S required adrenaline as cofactor. A 80 % ethanol precipitate of crude skin extract was fractionated through Sephadex G-100 column chromatography. The activity of G2-chalone existed in the low molecular weight fraction (10, 000<MW<100, 000) and that of G1-chalone existed in the high molecular weight fraction (MW>100, 000). departmental bulletin paper 岩手大学教育学部 1982-10-30 VoR application/pdf 岩手大学教育学部研究年報 = Annual report of the Faculty of Education, University of Iwate 1 42 55 63 AN00017940 0367-7370 https://iwate-u.repo.nii.ac.jp/record/11869/files/erar-v42n1p55-63.pdf https://doi.org/10.15113/00011863 https://iwate-u.repo.nii.ac.jp/records/11869 eng