2026-03-12T16:04:23Z https://iwate-u.repo.nii.ac.jp/oai

oai:iwate-u.repo.nii.ac.jp:00015507 2023-05-15T13:02:32Z 1515:1519

KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media KATAYAMA, Masafumi ONUMA, Manabu FUKUDA, Tomokazu cell culture cell growth cellular senescence chick fibroblasts DNA damage growth medium Cultured cells are a useful resource for poultry scientists, since these cells allow scientists to evaluate biological responses to conditions such as infectious diseases in vitro while mimicking the whole-body response in birds. However avian cell culture requires an optimized basal medium, and there are currently relatively few options for this basal medium (medium 199 and KAv-1). This means that there is still room for the development of an optimal basal medium for avian cell culture. Here we compare KAv-1 medium, Dulbecco's modified Eagle medium (DMEM) and medium 199 during the culture of chick fibroblasts and determine that KAv-1 remains the optimal medium for these assays. Our results show that DNA damage is reduced in fibroblasts cultured in the KAv-1 medium, when compared to both DMEM and Medium 199 and that these cells also display improved growth dynamics in KAv-1 medium when compared to both DMEM and medium 199. To the best of our knowledge, this is the first study to describe a comparative analysis of culture media for avian cells, which would provide useful information for poultry scientists. journal article Japan Poultry Science Association 2021 VoR application/pdf The journal of poultry science 4 58 270 279 AA11564513 AA12018767 13490486 13467395 https://iwate-u.repo.nii.ac.jp/record/15507/files/jps-v58n4p270-279.pdf https://iwate-u.repo.nii.ac.jp/records/15507 eng info:doi/10.2141/jpsa.0200085 Ⓒ 2021 Japan Poultry Science Association https://creativecommons.org/licenses/by-nc-sa/4.0/