2026-03-07T15:37:56Z https://iwate-u.repo.nii.ac.jp/oai

oai:iwate-u.repo.nii.ac.jp:02001313 2025-12-22T02:16:34Z 1515:1522 1523:2083

高校生のPCR体験実習に向けたウミホタルのCOII遺伝子の解析 Analysis of Vargula hilgendorfii COII gene for hands-on PCR training for high school students 藤原, 歩 FUJIWARA, Ayumi 安川, 洋生 YASUKAWA, Hiro We amplified and analyzed the nucleotide sequence of the gene for the mitochondrial cytochrome c oxidase subunit II (COII gene; 702 bp) of sea firefly Vargula hilgendorfii collected at Hekinan City, Aichi Prefecture. The results obtained from 15 specimens showed that the sequences of the gene in eight specimens were completely identical, while those in seven had base substitutions. The results also showed that the base substitutions were found in 10 sites in the gene, two of which changed codons resulting in amino acid substitutions. Vargula hilgendorfii would be useful material for learning about PCR amplification, mutation and genetic variation. conference paper 大学共同利用機関法人自然科学研究機構基礎生物学研究所技術課 大学共同利用機関法人自然科学研究機構生理学研究所技術課 2024-07-08 VoR application/pdf 生物学技術研究会報告第35号 ; 生理学技術研究会報告第46号 30 33 1341-5727 0285-3299 https://iwate-u.repo.nii.ac.jp/record/2001313/files/ssgh-2024p30-33.pdf https://iwate-u.repo.nii.ac.jp/records/2001313 jpn https://biogiken.nibb.ac.jp/%E3%83%9B%E3%83%BC%E3%83%A0/%E6%8A%80%E8%A1%93%E7%A0%94%E7%A9%B6%E4%BC%9A%E5%A0%B1%E5%91%8A%E3%83%80%E3%82%A6%E3%83%B3%E3%83%AD%E3%83%BC%E3%83%89 open access