{"created":"2023-05-15T12:05:33.418970+00:00","id":10243,"links":{},"metadata":{"_buckets":{"deposit":"9798142c-2b07-4011-b62c-9d9d8f0d63c1"},"_deposit":{"created_by":3,"id":"10243","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10243"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00010243","sets":["1515:1519"]},"author_link":["63427","63425","63426"],"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-08-23","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageStart":"e42907","bibliographicVolumeNumber":"7","bibliographic_titles":[{"bibliographic_title":"PLOS ONE"}]}]},"item_16_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2013-01-29"}]},"item_16_description_12":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"We developed a mathematical model of a hypothetical neuronal signal transduction pathway to better understand olfactory perception in Caenorhabditis elegans. This worm has only three pairs of olfactory receptor neurons. Intracellular Ca2+ decreases in one pair of olfactory neurons in C. elegans, the AWC neurons, following application of an attractive odor and there is a transient increase in intracellular Ca2+ following removal of odor. The magnitude of this increase is positively correlated with the duration of odor stimulation. Additionally, this Ca2+ transient is induced by a cGMP second messenger system. We identified likely candidates for the signal transduction molecules functioning in this system based on available gene expression and physiological data from AWCs. Our model incorporated a G-protein-coupled odor receptor, a G-protein, a guanylate cyclase as the G-protein effector, and a single phosphodiesterase. Additionally, a cyclic-nucleotide-gated ion channel and a voltage-gated ion channel that mediated calcium influx were incorporated into the model. We posited that, upon odor stimulation, guanylate cyclase was suppressed by the G-protein and that, upon cessation of the stimulus, the G-protein–induced suppression ceased and cGMP synthesis was restored. A key element of our model was a Ca2+-dependent negative feedback loop that ensured that the calcium increases were transient. Two guanylate cyclase-activating proteins acted on the effector guanylate cyclase to negatively regulate cGMP signaling and the resulting calcium influx. Our model was able to closely replicate in silico three important features of the calcium dynamics of AWCs. Specifically, in our simulations, [Ca2+] increased rapidly and reached its peak within 10 s after the odor stimulus was removed, peak [Ca2+] increased with longer odor exposure, and [Ca2+] decreased during a second stimulus that closely followed an initial stimulus. However, application of random background signal (‘noise’) showed that certain components of the pathway were particularly sensitive to this noise.","subitem_description_type":"Other"}]},"item_16_publisher_14":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_16_relation_26":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0042907","subitem_relation_type_select":"DOI"}}]},"item_16_rights_18":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© Usuyama et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_16_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1932-6203","subitem_source_identifier_type":"ISSN"}]},"item_16_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"Laboratory of Bioscience, Faculty of Engineering, Iwate University"},{"subitem_text_value":"The United Graduate School of Agricultural Sciences, Iwate University"},{"subitem_text_value":"Laboratory of Bioscience, Faculty of Engineering, Iwate University"}]},"item_16_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Usuyama, Mamoru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ushida, Chisato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shingai, Ryuzo"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-11-14"}],"displaytype":"detail","filename":"ploso-v7i8pe42907.pdf","filesize":[{"value":"882.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ploso-v7i8pe42907.pdf","url":"https://iwate-u.repo.nii.ac.jp/record/10243/files/ploso-v7i8pe42907.pdf"},"version_id":"94eafc87-1ac9-4bc2-b5ef-fa6cf66a01d7"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A Model of the Intracellular Response of an Olfactory Neuron in Caenorhabditis elegans to Odor Stimulation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A Model of the Intracellular Response of an Olfactory Neuron in Caenorhabditis elegans to Odor Stimulation"}]},"item_type_id":"16","owner":"3","path":["1519"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-01-29"},"publish_date":"2013-01-29","publish_status":"0","recid":"10243","relation_version_is_last":true,"title":["A Model of the Intracellular Response of an Olfactory Neuron in Caenorhabditis elegans to Odor Stimulation"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-16T11:41:00.556382+00:00"}