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Establishment of monocular-limited photoreceptor degeneration models in rabbits
https://iwate-u.repo.nii.ac.jp/records/10369
https://iwate-u.repo.nii.ac.jp/records/103696ed9cf1f-1cbd-4b3c-b67f-bd3e3acfeabd
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
bmco-v13i19.pdf (1.3 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||
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| 公開日 | 2015-02-19 | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | Establishment of monocular-limited photoreceptor degeneration models in rabbits | |||||||||||||||
| 言語 | ||||||||||||||||
| 言語 | eng | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | Photoreceptor degeneration | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | Monocular | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | Pigmented rabbits | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | Verteporfin | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | Nitric oxide | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
| 資源タイプ | journal article | |||||||||||||||
| 著者 |
Isago, Hitomi
× Isago, Hitomi
× Sugano, Eriko
× Murayama, Namie
× Tamai, Makoto
× Tomita, Hiroshi
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| 著者(機関) | ||||||||||||||||
| 値 | Laboratory of Visual Neuroscience, Department of Chemistry and Bioengineering, Iwate University Graduate School of Engineering | |||||||||||||||
| 著者(機関) | ||||||||||||||||
| 値 | Laboratory of Visual Neuroscience, Department of Chemistry and Bioengineering, Iwate University Graduate School of Engineering | |||||||||||||||
| 著者(機関) | ||||||||||||||||
| 値 | Laboratory of Visual Neuroscience, Department of Chemistry and Bioengineering, Iwate University Graduate School of Engineering | |||||||||||||||
| 著者(機関) | ||||||||||||||||
| 値 | Tohoku University Graduate School of Medicine | |||||||||||||||
| 著者(機関) | ||||||||||||||||
| 値 | Laboratory of Visual Neuroscience, Department of Chemistry and Bioengineering, Iwate University Graduate School of Engineering, Clinical Research, Innovation and Education Center, Tohoku University Hospital | |||||||||||||||
| 登録日 | ||||||||||||||||
| 日付 | 2015-02-19 | |||||||||||||||
| 書誌情報 |
BMC Ophthalmology 巻 13, 号 19, 発行日 2013-05-01 |
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| Abstract | ||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||
| 内容記述 | Background:Numerous rodent models of photoreceptor degeneration have been developed for the study of visual function. However, no viable model has been established in a species that is more closely related to Homo sapiens. Here, we present a rabbit model of monocular photoreceptor degeneration. Methods:We tested 2 chemicals, verteporfin and sodium nitroprusside (SNP), for developing a 1-eye limited photoreceptor degeneration model in pigmented rabbits. After the intravenous injection of verteporfin, the retina was exposed to light from a halogen lamp for 0, 10, 30, or 60 min. Alternately, 100 μL of various concentrations of sodium nitroprusside (0.1 mM, 0.5 mM, and 1 mM) were intravitreously injected into the rabbit eye. Retinal degeneration was evaluated by fundus photography, electroretinogram (ERG), and histological examinations. Results:Fundus photographs of animals in the verteporfin- or SNP-treated groups showed evidence of retinal degeneration. The severity of this degradation depended on the duration of light exposure and the concentration of SNP administered. The degeneration was clearly limited to the light-exposed areas in the verteporfin-treated groups. Extensive retinal atrophy was observed in the SNP-treated groups. The a- and b-wave amplitudes were dramatically decreased on the ERGs from SNP-treated groups. Histological examination revealed that either verteporfin or SNP induced severe photoreceptor degeneration. High-dose SNP treatment (1 mM) was also associated with inner retinal layer degeneration. Conclusions:Both SNP and verteporfin clearly caused photoreceptor degeneration without any effect on the contralateral eye. These compounds therefore represent valuable tools for the empirical investigation of visual function recovery. The findings will inform guidelines for clinical applications such as retinal prostheses, cell-based therapy, and gene therapy. |
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| 出版者 | ||||||||||||||||
| 出版者 | BioMed Central Ltd | |||||||||||||||
| 権利 | ||||||||||||||||
| 権利情報 | © 2013 Isago et al.; licensee BioMed Central Ltd. | |||||||||||||||
| 権利URI | ||||||||||||||||
| 権利情報 | http://creativecommons.org/licenses/by/2.0 | |||||||||||||||
| DOI | ||||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||
| 関連識別子 | 10.1186/1471-2415-13-19 | |||||||||||||||
| 著者版フラグ | ||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||
