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Separation of cell cycle phase-specific chalones using the epidermal organ culture as an assay system
https://doi.org/10.15113/00011863
https://doi.org/10.15113/0001186307ea99aa-fdf2-4679-987f-54515668079a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
erar-v42n1p55-63.pdf (153.7 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2008-03-03 | |||||||||
| タイトル | ||||||||||
| タイトル | Separation of cell cycle phase-specific chalones using the epidermal organ culture as an assay system | |||||||||
| その他の言語のタイトル | ||||||||||
| その他のタイトル | 器官培養系での表皮キヤロンの細胞分裂およびDNA合成に対する効果 | |||||||||
| 著者 |
HIROBE, Tomohisa
× HIROBE, Tomohisa
× YAMAGUCHI, Takeo
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| 著者別名 | ||||||||||
| 姓名 | 広部, 知久 | |||||||||
| 著者別名 | ||||||||||
| 姓名 | 山口, 武雄 | |||||||||
| 著者(機関) | ||||||||||
| Faculty of Education, Department of Biology Iwate University | ||||||||||
| 著者(機関) | ||||||||||
| National Institute of Radiological Sciences, Division of Biology | ||||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | assay system | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | epidermal organ culture | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | cell cycle phase-specific chalones | |||||||||
| Abstract | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | When a piece of mouse ear conch was laid on a disk of glass filter immersed in a TC 199 medium and kept at 37°C in an atmosphere of 95 % O2+5 % CO2 gas mixture, an abrupt increase in the number of mitoses and DNA synthesizing cells was observed between 12 and 24 h after the initiation of culture. The skin extract containing epidermal chalone was added to the culture medium at the time on the course of increase in mitoses and DNA synthesizing cells. In the case of DNA synthesizing cells 3H-thymidine was added various times later. Both G2-chalone which inhibited the transition from G2 to M and G1-chalone which inhibited the transition from G1 to S required adrenaline as cofactor. A 80 % ethanol precipitate of crude skin extract was fractionated through Sephadex G-100 column chromatography. The activity of G2-chalone existed in the low molecular weight fraction (10, 000<MW<100, 000) and that of G1-chalone existed in the high molecular weight fraction (MW>100, 000). | |||||||||
| 出版者 | ||||||||||
| 出版者 | 岩手大学教育学部 | |||||||||
| 登録日 | ||||||||||
| 日付 | 2008-03-03 | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| ID登録 | ||||||||||
| ID登録 | 10.15113/00011863 | |||||||||
| ID登録タイプ | JaLC | |||||||||
| NCID | ||||||||||
| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AN00017940 | |||||||||
| 書誌情報 |
岩手大学教育学部研究年報 = Annual report of the Faculty of Education, University of Iwate 巻 42, 号 1, p. 55-63, 発行日 1982-10-30 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 0367-7370 | |||||||||
