{"created":"2023-05-15T12:09:52.625397+00:00","id":15464,"links":{},"metadata":{"_buckets":{"deposit":"1f808d1d-eb9a-4798-aaec-a64caeccd9e8"},"_deposit":{"created_by":19,"id":"15464","owners":[19],"pid":{"revision_id":0,"type":"depid","value":"15464"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00015464","sets":["1504:1510"]},"author_link":["93368","93367","93365","93369","93370","93366"],"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-09-04","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"488","bibliographicVolumeNumber":"3","bibliographic_titles":[{"bibliographic_title":"Communications Biology"}]}]},"item_16_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2021-12-07"}]},"item_16_description_11":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Picorna-like plant viruses are non-enveloped RNA spherical viruses of ~30 nm. Part of the survival of these viruses depends on their capsid being stable enough to harbour the viral genome and yet malleable enough to allow its release. However, molecular mechanisms remain obscure. Here, we report a structure of a picorna-like plant virus, apple latent spherical virus, at 2.87 Å resolution by single-particle cryo-electron microscopy (cryo-EM) with a cold-field emission beam. The cryo-EM map reveals a unique structure composed of three capsid proteins Vp25, Vp20, and Vp24. Strikingly Vp25 has a long N-terminal extension, which substantially stabilises the capsid frame of Vp25 and Vp20 subunits. Cryo-EM images also resolve RNA genome leaking from a pentameric protrusion of Vp24 subunits. The structures and observations suggest that genome release occurs through occasional opening of the Vp24 subunits, possibly suppressed to a low frequency by the rigid frame of the other subunits.","subitem_description_type":"Abstract"}]},"item_16_publisher_14":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature"}]},"item_16_relation_17":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Apple latent spherical virus structure with stable capsid frame supports quasi-stable protrusions expediting genome release"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.nature.com/articles/s42003-020-01217-4","subitem_relation_type_select":"URI"}}]},"item_16_relation_26":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1038/s42003-020-01217-4","subitem_relation_type_select":"DOI"}}]},"item_16_rights_18":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2020"}]},"item_16_rights_19":{"attribute_name":"権利URI","attribute_value_mlt":[{"subitem_rights":"http://creativecommons.org/licenses/by/4.0/"}]},"item_16_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"23993642","subitem_source_identifier_type":"ISSN"}]},"item_16_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"Biostructural Mechanism Laboratory, RIKEN SPring-8 Center"},{"subitem_text_value":"Biostructural Mechanism Laboratory, RIKEN SPring-8 Center"},{"subitem_text_value":"Biostructural Mechanism Laboratory, RIKEN SPring-8 Center"},{"subitem_text_value":"Plant Pathology Laboratory, Faculty of Agriculture, Iwate University"},{"subitem_text_value":"Agri-Innovation Center, Iwate University"},{"subitem_text_value":"Biostructural Mechanism Laboratory, RIKEN SPring-8 Center"},{"subitem_text_value":"Biostructural Mechanism Laboratory, RIKEN SPring-8 Center, Advanced Electron Microscope Development Unit, RIKEN-JEOL Collaboration Center, RIKEN Baton Zone Program"}]},"item_16_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"NAITO, Hisashi"}],"nameIdentifiers":[{"nameIdentifier":"93365","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"HAMAGUCHI, Tasuku"}],"nameIdentifiers":[{"nameIdentifier":"93366","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"MAKI-YONEKURA, Saori "}],"nameIdentifiers":[{"nameIdentifier":"93367","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ISOGAI, Masamichi"}],"nameIdentifiers":[{"nameIdentifier":"93368","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"YOSHIKAWA, Nobuyuki"}],"nameIdentifiers":[{"nameIdentifier":"93369","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"YONEKURA, Koji"}],"nameIdentifiers":[{"nameIdentifier":"93370","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-12-07"}],"displaytype":"detail","filename":"cv-v3p488.pdf","filesize":[{"value":"3.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"cv-v3p488","url":"https://iwate-u.repo.nii.ac.jp/record/15464/files/cv-v3p488.pdf"},"version_id":"6b21ab5d-0aa1-4b18-9e7f-06a5693c998a"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Apple latent spherical virus structure with stable capsid frame supports quasi-stable protrusions expediting genome release","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Apple latent spherical virus structure with stable capsid frame supports quasi-stable protrusions expediting genome release"}]},"item_type_id":"16","owner":"19","path":["1510"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-12-07"},"publish_date":"2021-12-07","publish_status":"0","recid":"15464","relation_version_is_last":true,"title":["Apple latent spherical virus structure with stable capsid frame supports quasi-stable protrusions expediting genome release"],"weko_creator_id":"19","weko_shared_id":-1},"updated":"2023-05-15T13:04:13.213304+00:00"}