| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2021-12-09 |
| タイトル |
|
|
タイトル |
Apple latent spherical virus (ALSV)‑induced gene silencing in a medicinal plant, Lithospermum erythrorhizon |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
IZUISHI, Yuki
ISAKA, Natsumi
LI, Hao
NAKANISHI, Kohei
KAGEYAMA, Joji
ISHIKAWA, Kazuya
SHIMADA, Tomoo
MASUTA, Chikara
YOSHIKAWA, Nobuyuki
KUSANO, Hiroaki
YAZAKI, Kazufumi
|
| 著者(機関) |
|
|
値 |
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University |
| 著者(機関) |
|
|
値 |
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University |
| 著者(機関) |
|
|
値 |
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University |
| 著者(機関) |
|
|
値 |
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University |
| 著者(機関) |
|
|
値 |
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University |
| 著者(機関) |
|
|
値 |
Graduate School of Science, Kyoto University |
| 著者(機関) |
|
|
値 |
Graduate School of Science, Kyoto University |
| 著者(機関) |
|
|
値 |
Research Faculty of Agriculture, Hokkaido University |
| 著者(機関) |
|
|
値 |
Agri‑Innovation Center, Iwate University |
| 著者(機関) |
|
|
値 |
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University |
| 著者(機関) |
|
|
値 |
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University |
| 登録日 |
|
|
日付 |
2021-12-09 |
| 書誌情報 |
Scientific Reports
巻 10,
p. 13555,
発行日 2020-08-11
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20452322 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Lithospermum erythrorhizon is a medicinal plant that produces shikonin, a red lipophilic naphthoquinone derivative that accumulates exclusively in roots. The biosynthetic steps required to complete the naphthalene ring of shikonin and its mechanism of secretion remain unclear. Multiple omics studies identified several candidate genes involved in shikonin production. The functions of these genes can be evaluated using virus-induced gene silencing (VIGS) systems, which have been shown advantageous in introducing iRNA genes into non-model plants. This study describes the development of a VIGS system using an apple latent spherical virus (ALSV) vector and a target gene, phytoene desaturase (LePDS1). Virus particles packaged in Nicotiana benthamiana were inoculated into L. erythrorhizon seedlings, yielding new leaves with albino phenotype but without disease symptoms. The levels of LePDS1 mRNAs were significantly lower in the albino plants than in mock control or escape plants. Virus-derived mRNA was detected in infected plants but not in escape and mock plants. Quantitative PCR and deep sequencing analysis indicated that transcription of another hypothetical PDS gene (LePDS2) also decreased in the defective leaves. Virus infection, however, had no effect on shikonin production. These results suggest that virus-based genetic transformation and the VIGS system silence target genes in soil-grown L. erythrorhizon. |
| 出版者 |
|
|
出版者 |
Springer Nature |
| 関係URI |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
https://www.nature.com/articles/s41598-020-70469-1 |
|
|
関連名称 |
Apple latent spherical virus (ALSV)‑induced gene silencing in a medicinal plant, Lithospermum erythrorhizon |
| 権利 |
|
|
権利情報 |
© The Author(s) 2020 |
| 権利URI |
|
|
権利情報 |
http://creativecommons.org/licenses/by/4.0/ |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
info:doi/10.1038/s41598-020-70469-1 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
sr-v10p13555 (3.9 MB)
