{"created":"2023-05-15T12:09:53.065811+00:00","id":15475,"links":{},"metadata":{"_buckets":{"deposit":"4b9156ee-abac-4845-a395-e1bcbec564be"},"_deposit":{"created_by":19,"id":"15475","owners":[19],"pid":{"revision_id":0,"type":"depid","value":"15475"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00015475","sets":["1504:1510"]},"author_link":["93454","93451","93449","93448","93450","93453","93445","93447","93455","93444","93446","93443","93452"],"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-02-04","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"1372","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"Scientific Reports"}]}]},"item_16_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2021-12-13"}]},"item_16_description_11":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"MPIase is a glycolipid that is involved in membrane protein integration. Despite evaluation of its functions in vitro, the lack of information on MPIase biosynthesis hampered verification of its involvement in vivo. In this study, we found that depletion of CdsA, a CDP-diacylglycerol synthase, caused not only a defect in phospholipid biosynthesis but also MPIase depletion with accumulation of the precursors of both membrane protein M13 coat protein and secretory protein OmpA. Yeast Tam41p, a mitochondrial CDP-diacylglycerol synthase, suppressed the defect in phospholipid biosynthesis, but restored neither MPIase biosynthesis, precursor processing, nor cell growth, indicating that MPIase is essential for membrane protein integration and therefore for cell growth. Consistently, we observed a severe defect in protein integration into MPIase-depleted membrane vesicles in vitro. Thus, the function of MPIase as a factor involved in protein integration was proven in vivo as well as in vitro. Moreover, Cds1p, a eukaryotic CdsA homologue, showed a potential for MPIase biosynthesis. From these results, we speculate the presence of a eukaryotic MPIase homologue.","subitem_description_type":"Abstract"}]},"item_16_publisher_14":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature"}]},"item_16_relation_17":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"CdsA is involved in biosynthesis of glycolipid MPIase essential for membrane protein integration in vivo"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.nature.com/articles/s41598-018-37809-8","subitem_relation_type_select":"URI"}}]},"item_16_relation_26":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1038/s41598-018-37809-8","subitem_relation_type_select":"DOI"}}]},"item_16_rights_18":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2019"}]},"item_16_rights_19":{"attribute_name":"権利URI","attribute_value_mlt":[{"subitem_rights":"http://creativecommons.org/licenses/by/4.0/"}]},"item_16_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"20452322","subitem_source_identifier_type":"ISSN"}]},"item_16_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"The United Graduate School of Agricultural Sciences, Iwate University"},{"subitem_text_value":"The United Graduate School of Agricultural Sciences, Iwate University"},{"subitem_text_value":"The United Graduate School of Agricultural Sciences, Iwate University"},{"subitem_text_value":"Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University"},{"subitem_text_value":"Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University"},{"subitem_text_value":"Bioorganic Research Institute, Suntory Foundation for Life Sciences"},{"subitem_text_value":"Bioorganic Research Institute, Suntory Foundation for Life Sciences"},{"subitem_text_value":"Earth-Life Science Institute, Tokyo Institute of Technology"},{"subitem_text_value":"Faculty of Science, Yamagata University, Yamagata"},{"subitem_text_value":"Faculty of Life Sciences, Kyoto Sangyo University"},{"subitem_text_value":"Department of Computational Biology and Medical Sciences Graduate School of Frontier Sciences"},{"subitem_text_value":"Bioorganic Research Institute, Suntory Foundation for Life Sciences"},{"subitem_text_value":"The United Graduate School of Agricultural Sciences, Iwate University, Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University, Department of Biological Chemistry and Food Science, Faculty of Agriculture, Iwate University"}]},"item_16_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SAWASATO, Katsuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SATO, Ryo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"NISHIKAWA, Hanako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"IIMURA, Naoki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KAMEMOTO, Yuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"FUJIKAWA, Kohki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YAMAGUCHI, Toshiyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KURUMA, Yutetsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TAMURA, Yasushi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ENDO, Toshiya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"UEDA, Takuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SHIMAMOTO, Keiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"NISHIYAMA, Ken-ichi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-12-13"}],"displaytype":"detail","filename":"sr-v9p1372.pdf","filesize":[{"value":"4.9 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vivo"}]},"item_type_id":"16","owner":"19","path":["1510"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-12-13"},"publish_date":"2021-12-13","publish_status":"0","recid":"15475","relation_version_is_last":true,"title":["CdsA is involved in biosynthesis of glycolipid MPIase essential for membrane protein integration in vivo"],"weko_creator_id":"19","weko_shared_id":-1},"updated":"2023-05-15T13:03:52.780449+00:00"}