{"created":"2023-05-15T12:09:53.377518+00:00","id":15484,"links":{},"metadata":{"_buckets":{"deposit":"2ca6bb4c-de3e-4834-93e2-b68c4fb7d7d5"},"_deposit":{"created_by":19,"id":"15484","owners":[19],"pid":{"revision_id":0,"type":"depid","value":"15484"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00015484","sets":["1515:1519"]},"author_link":["93510","93512","93513","93511","93516","93514","93509","93515"],"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-01-23","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"41210","bibliographicVolumeNumber":"7","bibliographic_titles":[{"bibliographic_title":"Scientific Reports"}]}]},"item_16_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2021-12-15"}]},"item_16_description_11":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Optogenetic technologies are expected to be applicable for clinical use in restoring vision. However, the degree of recovered visual function is highly dependent on the function of the chosen optogenetic gene. To investigate the effect on visual function of dual expression of genes with different wavelength sensitivities, we transduced a modified Volvox-derived channelrhodopsin gene (mVChR1) via an adeno-associated virus vector into transgenic rats harbouring the ChR2 gene in retinal ganglion cells. These transgenic rats were given an intraperitoneal injection of N-methyl-N-nitrosourea to induce the degeneration of native photoreceptor cells prior to transduction of mVChR1. Optical coherence tomography images indicated the degeneration of the native photoreceptor cells after the N-methyl-N-nitrosourea injection. Complete loss of function of the native photoreceptor cells was confirmed using electroretinograms. In the ChR2 transgenic rats, visually evoked potentials were clearly detectable in spite of native photoreceptor function abolishment; however the responses were limited to within blue wavelengths. In contrast, the limited wavelength sensitivities were improved by the additional transduction of mVChR1, which exhibited sensitivities to green and red. Thus, the transductions of dual genes encoding channelrhodopsins that exhibit different wavelength sensitivities represents a promising candidate method to expand and to enhance rescued wavelength sensitivities in blind subjects.","subitem_description_type":"Abstract"}]},"item_16_publisher_14":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature"}]},"item_16_relation_17":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Visual Responses of Photoreceptor- Degenerated Rats Expressing Two Different Types of Channelrhodopsin Genes"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.nature.com/articles/srep41210","subitem_relation_type_select":"URI"}}]},"item_16_relation_26":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1038/srep41210","subitem_relation_type_select":"DOI"}}]},"item_16_rights_18":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2017"}]},"item_16_rights_19":{"attribute_name":"権利URI","attribute_value_mlt":[{"subitem_rights":"http://creativecommons.org/licenses/by/4.0/"}]},"item_16_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"20452322","subitem_source_identifier_type":"ISSN"}]},"item_16_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"Laboratory of Visual Neuroscience, Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering"},{"subitem_text_value":"Laboratory of Visual Neuroscience, Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering, Soft-Path Engineering Research Center (SPERC), Iwate University Division of Science and Engineering"},{"subitem_text_value":"Laboratory of Visual Neuroscience, Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering"},{"subitem_text_value":"Laboratory of Visual Neuroscience, Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering"},{"subitem_text_value":"Laboratory of Visual Neuroscience, Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering"},{"subitem_text_value":"Laboratory of Visual Neuroscience, Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering, Soft-Path Engineering Research Center (SPERC), Iwate University Division of Science and Engineering"},{"subitem_text_value":"Tohoku University Graduate School of Medicine"},{"subitem_text_value":"Laboratory of Visual Neuroscience, Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering, Soft-Path Engineering Research Center (SPERC), Iwate University Division of Science and Engineering, Clinical Research, Innovation and Education Center, Tohoku University Hospital"}]},"item_16_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SATO, Masatoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SUGANO, Eriko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TABATA, Kitako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SANNOHE, Kei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"WATANABE, Yoshito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OZAKI, Taku"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TAMAI, Makoto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TOMITA, Hiroshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-12-15"}],"displaytype":"detail","filename":"sr-v7p41210.pdf","filesize":[{"value":"2.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"sr-v7p41210","url":"https://iwate-u.repo.nii.ac.jp/record/15484/files/sr-v7p41210.pdf"},"version_id":"12b97620-9cae-419b-98fa-e2dea39dd9c4"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Visual Responses of Photoreceptor-Degenerated Rats Expressing Two Different Types of Channelrhodopsin Genes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Visual Responses of Photoreceptor-Degenerated Rats Expressing Two Different Types of Channelrhodopsin Genes"}]},"item_type_id":"16","owner":"19","path":["1519"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-12-15"},"publish_date":"2021-12-15","publish_status":"0","recid":"15484","relation_version_is_last":true,"title":["Visual Responses of Photoreceptor-Degenerated Rats Expressing Two Different Types of Channelrhodopsin Genes"],"weko_creator_id":"19","weko_shared_id":-1},"updated":"2023-05-15T13:03:32.510731+00:00"}