@article{oai:iwate-u.repo.nii.ac.jp:00015489,
 author = {ONDA, Yoshihiko and MOCHIDA, Keiichi and YOSHIDA, Takuhiro and SAKURAI, Tetsuya and SEYMOUR, Roger S. and UMEKAWA, Yui and Pirintsos, Stergios Arg and SHINOZAKI, Kazuo and ITO, Kikukatsu},
 journal = {Scientific Reports},
 month = {Mar},
 note = {Several plant species can generate enough heat to increase their internal floral temperature above ambient temperature. Among thermogenic plants, Arum concinnatum shows the highest respiration activity during thermogenesis. However, an overall understanding of the genes related to plant thermogenesis has not yet been achieved. In this study, we performed de novo transcriptome analysis of flower organs in A. concinnatum. The de novo transcriptome assembly represented, in total, 158,490 non-redundant transcripts and 53,315 of those showed significant homology with known genes. To explore genes associated with thermogenesis, we filtered 1266 transcripts that showed a significant correlation between expression pattern and the temperature trend of each sample. We confirmed five putative alternative oxidase transcripts were included in filtered transcripts as expected. An enrichment analysis of the Gene Ontology terms for the filtered transcripts suggested over-representation of genes involved in 1-deoxy-d-xylulose-5-phosphate synthase (DXS) activity. The expression profiles of DXS transcripts in the methyl-d-erythritol 4-phosphate (MEP) pathway were significantly correlated with thermogenic levels. Our results suggest that the MEP pathway is the main biosynthesis route for producing scent monoterpenes. To our knowledge, this is the first report describing the candidate pathway and the key enzyme for floral scent production in thermogenic plants.},
 title = {Transcriptome analysis of thermogenic Arum concinnatum reveals the molecular components of floral scent production},
 volume = {5},
 year = {2015}
}