{"created":"2023-05-15T12:09:53.868719+00:00","id":15495,"links":{},"metadata":{"_buckets":{"deposit":"aa1364d8-d40b-4f84-8cc3-29df49e23174"},"_deposit":{"created_by":19,"id":"15495","owners":[19],"pid":{"revision_id":0,"type":"depid","value":"15495"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00015495","sets":["1504:1510","1515:1519"]},"author_link":["93619","93616","93617","93614","93618","93615","93613"],"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-09-17","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"17","bibliographicPageEnd":"3431","bibliographicPageStart":"3417","bibliographicVolumeNumber":"477","bibliographic_titles":[{"bibliographic_title":"Biochemical Journal"}]}]},"item_16_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2021-12-27"}]},"item_16_description_11":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cyanide-resistant alternative oxidase (AOX) is a nuclear-encoded quinol oxidase located in the inner mitochondrial membrane. Although the quality control of AOX proteins is expected to have a role in elevated respiration in mitochondria, it remains unclear whether thermogenic plants possess molecular mechanisms for the mitochondrial degradation of AOX. To better understand the mechanism of AOX turnover in mitochondria, we performed a series of in organello AOX degradation assays using mitochondria from various stages of the appendices of Arum maculatum. Our analyses clearly indicated that AOX proteins at certain stages in the appendices are degraded at 30°C, which is close to the maximum appendix temperature observed during thermogenesis. Interestingly, such temperature-dependent protease activities were specifically inhibited by E-64, a cysteine protease inhibitor. Moreover, purification and subsequent nano LC–MS/MS analyses of E-64-sensitive and DCG-04-labeled active mitochondrial protease revealed an ∼30 kDa protein with an identical partial peptide sequence to the cysteine protease 1-like protein from Phoenix dactylifera. Our data collectively suggest that AOX is a potential target for temperature-dependent E-64-sensitive cysteine protease in the appendices of A. maculatum. A possible retrograde signalling cascade mediated by specific degradation of AOX proteins and its physiological significance are discussed.","subitem_description_type":"Abstract"}]},"item_16_publisher_14":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Portland Press"}]},"item_16_relation_17":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Degradation of mitochondrial alternative oxidase in the appendices of Arum maculatum"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://portlandpress.com/biochemj/article/477/17/3417/226215/Degradation-of-mitochondrial-alternative-oxidase","subitem_relation_type_select":"URI"}}]},"item_16_relation_26":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1042/BCJ20200515","subitem_relation_type_select":"DOI"}}]},"item_16_rights_18":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2020 The Author(s)"}]},"item_16_rights_19":{"attribute_name":"権利URI","attribute_value_mlt":[{"subitem_rights":"https://creativecommons.org/licenses/by/4.0/"}]},"item_16_source_id_25":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA12366617","subitem_source_identifier_type":"NCID"}]},"item_16_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"14708728","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"02646021","subitem_source_identifier_type":"ISSN"}]},"item_16_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"Faculty of Agriculture, Iwate University, Agri-Innovation Center, Iwate University, United Graduate School of Agricultural Science, Iwate University"},{"subitem_text_value":"Faculty of Agriculture, Iwate University"},{"subitem_text_value":"Faculty of Agriculture, Iwate University"},{"subitem_text_value":"United Graduate School of Agricultural Science, Iwate University"},{"subitem_text_value":"United Graduate School of Agricultural Science, Iwate University"},{"subitem_text_value":"Department of Electrical and Electronic Engineering, Faculty of Science and Engineering, Iwate University"},{"subitem_text_value":"Department of Biochemistry and Biomedicine, School of Life Sciences, University of Sussex"}]},"item_16_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ITO, Kikukatsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OGATA, Takafumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SEITO, Takanari"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"UMEKAWA, Yui"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KAKIZAKI, Yusuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OSADA, Hiroshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MOORE, Anthony L."}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-12-27"}],"displaytype":"detail","filename":"bj-v477n17p3417-3431.pdf","filesize":[{"value":"2.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bj-v477n17p3417-3431","url":"https://iwate-u.repo.nii.ac.jp/record/15495/files/bj-v477n17p3417-3431.pdf"},"version_id":"a4b88967-24b6-4f33-ae6b-846deec92844"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Degradation of mitochondrial alternative oxidase in the appendices of Arum maculatum","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Degradation of mitochondrial alternative oxidase in the appendices of Arum maculatum"}]},"item_type_id":"16","owner":"19","path":["1510","1519"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-12-27"},"publish_date":"2021-12-27","publish_status":"0","recid":"15495","relation_version_is_last":true,"title":["Degradation of mitochondrial alternative oxidase in the appendices of Arum maculatum"],"weko_creator_id":"19","weko_shared_id":-1},"updated":"2023-05-15T13:03:07.185196+00:00"}