@article{oai:iwate-u.repo.nii.ac.jp:00015500, author = {Kovács, Terézia and Mohamed Ahres and Pálmai, Tamás and Kovács, László and UEMURA, Matsuo and Crosatti, Cristina and Galiba, Gabor}, issue = {20}, journal = {International journal of molecular sciences}, month = {Oct}, note = {In cereals, C-repeat binding factor genes have been defined as key components of the light quality-dependent regulation of frost tolerance by integrating phytochrome-mediated light and temperature signals. This study elucidates the di_erences in the lipid composition of barley leaves illuminated with white light or white light supplemented with far-red light at 5 or 15 _C. According to LC-MS analysis, far-red light supplementation increased the amount of monogalactosyldiacylglycerol species 36:6, 36:5, and 36:4 after 1 day at 5 _C, and 10 days at 15 _C resulted in a perturbed content of 38:6 species. Changes were observed in the levels of phosphatidylethanolamine, and phosphatidylserine under white light supplemented with far-red light illumination at 15 _C, whereas robust changes were observed in the amount of several phosphatidylserine species at 5 _C. At 15 _C, the amount of some phosphatidylglycerol species increased as a result of white light supplemented with far-red light illumination after 1 day. The ceramide (42:2)-3 content increased regardless of the temperature. The double-bond index of phosphatidylglycerol, phosphatidylserine, phosphatidylcholine ceramide together with total double-bond index changed when the plant was grown at 15 _C as a function of white light supplemented with far-red light. white light supplemented with far-red light increased the monogalactosyldiacylglycerol/diacylglycerol ratio as well. The gene expression changes are well correlated with the alterations in the lipidome.}, title = {Decreased R:FR Ratio in Incident White Light Affects the Composition of Barley Leaf Lipidome and Freezing Tolerance in a Temperature-Dependent Manner}, volume = {21}, year = {2020} }