{"created":"2023-05-15T12:09:54.182188+00:00","id":15502,"links":{},"metadata":{"_buckets":{"deposit":"f6d7b5ef-3d39-4028-9b4c-846667c5d98a"},"_deposit":{"created_by":19,"id":"15502","owners":[19],"pid":{"revision_id":0,"type":"depid","value":"15502"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00015502","sets":["1504:1510"]},"author_link":["93649","93647","93648","93650"],"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-10-21","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"345","bibliographicPageStart":"339","bibliographicVolumeNumber":"24","bibliographic_titles":[{"bibliographic_title":"Plant Production Science"}]}]},"item_16_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2022-01-05"}]},"item_16_description_11":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Isolation of an adequate quantity of high-quality RNA is a crucial step in gene expression analysis. Difficulties in sampling and RNA extraction of field-grown crops, especially from roots, are substantial obstacles to understanding actual gene expression in the field. We examined the effectiveness of the RNA extraction method (FRP) using pot- and field-grown soybean roots. High quantity and quality of total RNA could extract by the FRP method from both pot- and field-grown soybean roots at different growth stages. To determine the influence of root washing during the sampling process, roots were washed with water for 5 s, 1 min, and 5 min, then analyzed for aquaporin gene expression levels. Root washing for 5 min affected gene expression of some aquaporins. To obtain RNA that reflects the correct gene expression profile, it is necessary to minimize the effects of the sampling process.","subitem_description_type":"Abstract"}]},"item_16_publisher_14":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Taylor & Francis Group"}]},"item_16_relation_17":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Root sampling and RNA extraction methods for field-based gene expression analysis of soybeans"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.tandfonline.com/doi/full/10.1080/1343943X.2020.1834867","subitem_relation_type_select":"URI"}}]},"item_16_relation_26":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1080/1343943X.2020.1834867","subitem_relation_type_select":"DOI"}}]},"item_16_rights_18":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group."}]},"item_16_rights_19":{"attribute_name":"権利URI","attribute_value_mlt":[{"subitem_rights":"http://creativecommons.org/licenses/by/4.0/"}]},"item_16_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1343943X","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"13491008","subitem_source_identifier_type":"ISSN"}]},"item_16_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"Department of Plant Bio Sciences, Faculty of Agriculture, Iwate University"},{"subitem_text_value":"Graduate School of Arts and Sciences, Division of Agriculture, Iwate University"},{"subitem_text_value":"Department of Plant Bio Sciences, Faculty of Agriculture, Iwate University, Agri-Innovation Center, Iwate University"},{"subitem_text_value":"Department of Plant Bio Sciences, Faculty of Agriculture, Iwate University"}]},"item_16_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"OKAMURA, Shin"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mumtahina, Nabila"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SHIMONO, Hiroyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MATSUNAMI, Maya"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-05"}],"displaytype":"detail","filename":"pps-v24n3p339-345.pdf","filesize":[{"value":"3.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"pps-v24n3p339-345","url":"https://iwate-u.repo.nii.ac.jp/record/15502/files/pps-v24n3p339-345.pdf"},"version_id":"de2686e5-bf41-4dca-8224-42c74373ca7a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Aquaporin","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA extraction","subitem_subject_scheme":"Other"},{"subitem_subject":"root","subitem_subject_scheme":"Other"},{"subitem_subject":"soybean","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Root sampling and RNA extraction methods for field-based gene expression analysis of soybeans","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Root sampling and RNA extraction methods for field-based gene expression analysis of soybeans"}]},"item_type_id":"16","owner":"19","path":["1510"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-01-05"},"publish_date":"2022-01-05","publish_status":"0","recid":"15502","relation_version_is_last":true,"title":["Root sampling and RNA extraction methods for field-based gene expression analysis of soybeans"],"weko_creator_id":"19","weko_shared_id":-1},"updated":"2023-05-15T13:02:50.857041+00:00"}