{"created":"2023-05-15T12:09:54.412428+00:00","id":15507,"links":{},"metadata":{"_buckets":{"deposit":"b2a280ed-d147-4f71-a980-e89ff178a7e8"},"_deposit":{"created_by":19,"id":"15507","owners":[19],"pid":{"revision_id":0,"type":"depid","value":"15507"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00015507","sets":["1515:1519"]},"author_link":["93682","93683","93684"],"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"279","bibliographicPageStart":"270","bibliographicVolumeNumber":"58","bibliographic_titles":[{"bibliographic_title":"The journal of poultry science"}]}]},"item_16_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2022-01-07"}]},"item_16_description_11":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cultured cells are a useful resource for poultry scientists, since these cells allow scientists to evaluate biological responses to conditions such as infectious diseases in vitro while mimicking the whole-body response in birds. However avian cell culture requires an optimized basal medium, and there are currently relatively few options for this basal medium (medium 199 and KAv-1). This means that there is still room for the development of an optimal basal medium for avian cell culture. Here we compare KAv-1 medium, Dulbecco's modified Eagle medium (DMEM) and medium 199 during the culture of chick fibroblasts and determine that KAv-1 remains the optimal medium for these assays. Our results show that DNA damage is reduced in fibroblasts cultured in the KAv-1 medium, when compared to both DMEM and Medium 199 and that these cells also display improved growth dynamics in KAv-1 medium when compared to both DMEM and medium 199. To the best of our knowledge, this is the first study to describe a comparative analysis of culture media for avian cells, which would provide useful information for poultry scientists.","subitem_description_type":"Abstract"}]},"item_16_link_13":{"attribute_name":"抄録（URL）","attribute_value_mlt":[{"subitem_link_text":"KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media","subitem_link_url":"https://www.jstage.jst.go.jp/article/jpsa/58/4/58_0200085/_article"}]},"item_16_publisher_14":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japan Poultry Science Association"}]},"item_16_relation_26":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.2141/jpsa.0200085","subitem_relation_type_select":"DOI"}}]},"item_16_rights_18":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Ⓒ 2021 Japan Poultry Science Association"}]},"item_16_rights_19":{"attribute_name":"権利URI","attribute_value_mlt":[{"subitem_rights":"https://creativecommons.org/licenses/by-nc-sa/4.0/"}]},"item_16_source_id_25":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA11564513","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA12018767","subitem_source_identifier_type":"NCID"}]},"item_16_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13490486","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"13467395","subitem_source_identifier_type":"ISSN"}]},"item_16_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, Wildlife Genome Collaborative Research Group, National Institute for Environmental Studies"},{"subitem_text_value":"Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, Wildlife Genome Collaborative Research Group, National Institute for Environmental Studies"},{"subitem_text_value":"Wildlife Genome Collaborative Research Group, National Institute for Environmental Studies, Graduate School of Science and Engineering"}]},"item_16_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"KATAYAMA, Masafumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ONUMA, Manabu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"FUKUDA, Tomokazu"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-07"}],"displaytype":"detail","filename":"jps-v58n4p270-279.pdf","filesize":[{"value":"3.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"jps-v58n4p270-279","url":"https://iwate-u.repo.nii.ac.jp/record/15507/files/jps-v58n4p270-279.pdf"},"version_id":"7118d038-3abf-4852-a243-76013652dc4b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cell culture","subitem_subject_scheme":"Other"},{"subitem_subject":"cell growth","subitem_subject_scheme":"Other"},{"subitem_subject":"cellular senescence","subitem_subject_scheme":"Other"},{"subitem_subject":"chick fibroblasts","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA damage","subitem_subject_scheme":"Other"},{"subitem_subject":"growth medium","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media"}]},"item_type_id":"16","owner":"19","path":["1519"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-01-07"},"publish_date":"2022-01-07","publish_status":"0","recid":"15507","relation_version_is_last":true,"title":["KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media"],"weko_creator_id":"19","weko_shared_id":-1},"updated":"2023-05-15T13:02:32.872779+00:00"}