{"created":"2023-05-15T12:09:54.456537+00:00","id":15508,"links":{},"metadata":{"_buckets":{"deposit":"c31d9fa0-bc89-420c-9912-6b2e0edf3d9f"},"_deposit":{"created_by":19,"id":"15508","owners":[19],"pid":{"revision_id":0,"type":"depid","value":"15508"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00015508","sets":["1504:1510"]},"author_link":["93687","93685","93686"],"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"262","bibliographicPageStart":"258","bibliographicVolumeNumber":"58","bibliographic_titles":[{"bibliographic_title":"The journal of poultry science"}]}]},"item_16_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2022-01-11"}]},"item_16_description_11":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Glycation is a non-enzymatic reaction, and amino acids are glycated by glucose in vivo. Tryptophan is glycated with glucose to form two types of glycated compounds, tryptophan-Amadori product and (1R, 3S)-1-(D-gluco-1, 2, 3, 4, 5-pentahydroxypentyl)-1, 2, 3, 4-tetrahydro-β-carboline-3-carboxylic acid (PHP-THβC). Although PHP-THβC can be incorporated into various chicken embryonic cells, the mechanism of its incorporation into intracellular fluids has not been clarified. In this study, we examined whether PHP-THβC once incorporated into various chicken embryonic cells can combine with proteins. Embryonic cells from the breast muscle, liver, spleen, kidney, proventriculus, gizzard, and skin were prepared and 3H-PHP-THβC was added to the culture medium at final concentrations of 0, 200, 400, 600, and 800 µM to examine the incorporation of PHP-THβC. After 18 h of incubation, radioactivity was measured in the whole-cell and protein fractions of the chicken embryonic cells. As PHP-THβC concentration increased from 0 to 600 µM, its accumulation in the whole-cell fractions of all types of chicken embryonic cells linearly increased and reached the maximum level. The saturated PHP-THβC accumulation in the whole-cell fractions suggests that PHP-THβC could be incorporated into intracellular fluids across cellular membranes by some transporter proteins. As PHP-THβC concentration increased from 0 to 800 µM, its accumulation in the protein fractions of all types of chicken embryonic cells increased in a linear manner and reached a maximum level in the 800 µM PHPTHβC treatment group. This is the first study to indicate that a part of PHP-THβC incorporated into the whole-cell fraction was detected in the protein fraction of various chicken embryonic cells.","subitem_description_type":"Abstract"}]},"item_16_link_13":{"attribute_name":"抄録（URL）","attribute_value_mlt":[{"subitem_link_text":"Glycated Tryptophan PHP-THβC Incorporated into Various Chicken Embryonic Cells Constitutes Cellular Proteins","subitem_link_url":"https://www.jstage.jst.go.jp/article/jpsa/58/4/58_0200076/_article"}]},"item_16_relation_26":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.2141/jpsa.0200076","subitem_relation_type_select":"DOI"}}]},"item_16_rights_18":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Ⓒ 2021 Japan Poultry Science Association"}]},"item_16_rights_19":{"attribute_name":"権利URI","attribute_value_mlt":[{"subitem_rights":"https://creativecommons.org/licenses/by-nc-sa/4.0/"}]},"item_16_source_id_25":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA12018767","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA11564513","subitem_source_identifier_type":"NCID"}]},"item_16_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13490486","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"13467395","subitem_source_identifier_type":"ISSN"}]},"item_16_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"Faculty of Agriculture, Iwate University, Faculty of Agriculture, Ehime University"},{"subitem_text_value":"Faculty of Agriculture, Iwate University, Graduate School of Systems Life Sciences, Kyushu University"},{"subitem_text_value":"Faculty of Agriculture, Iwate University"}]},"item_16_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MAKINO, Ryosuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ABE, Kayoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KITA, Kazumi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-11"}],"displaytype":"detail","filename":"jps-v58n4p258-262.pdf","filesize":[{"value":"310.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"jps-v58n4p258-262","url":"https://iwate-u.repo.nii.ac.jp/record/15508/files/jps-v58n4p258-262.pdf"},"version_id":"3352df9d-dc74-4997-89bd-0c001f33531d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"chicken embryo","subitem_subject_scheme":"Other"},{"subitem_subject":"glycation","subitem_subject_scheme":"Other"},{"subitem_subject":"PHP-THβC","subitem_subject_scheme":"Other"},{"subitem_subject":"protein synthesis","subitem_subject_scheme":"Other"},{"subitem_subject":"tryptophan","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Glycated Tryptophan PHP-THβC Incorporated into Various Chicken Embryonic Cells Constitutes Cellular Proteins","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Glycated Tryptophan PHP-THβC Incorporated into Various Chicken Embryonic Cells Constitutes Cellular Proteins"}]},"item_type_id":"16","owner":"19","path":["1510"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-01-11"},"publish_date":"2022-01-11","publish_status":"0","recid":"15508","relation_version_is_last":true,"title":["Glycated Tryptophan PHP-THβC Incorporated into Various Chicken Embryonic Cells Constitutes Cellular Proteins"],"weko_creator_id":"19","weko_shared_id":-1},"updated":"2023-05-15T13:02:31.567937+00:00"}