{"created":"2023-05-15T12:10:06.782594+00:00","id":15760,"links":{},"metadata":{"_buckets":{"deposit":"24b9850d-9317-42a7-b472-bf17a437d64b"},"_deposit":{"created_by":19,"id":"15760","owners":[19],"pid":{"revision_id":0,"type":"depid","value":"15760"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00015760","sets":["1504:1510"]},"author_link":["94920","94921","94922","94919"],"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"229","bibliographicPageStart":"226","bibliographicVolumeNumber":"83","bibliographic_titles":[{"bibliographic_title":"Journal of Veterinary Medical Science"}]}]},"item_16_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2022-08-17"}]},"item_16_description_11":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The poultry infections caused by Dispharynx nasuta and Cheilospirura hamulosa nematodes are difficult to be diagnosed by fecal examination because of their egg similarity. In this study, we analyzed DNA sequences of nuclear ribosomal 18S-ITS1-5.8S-ITS2-28S region of D. nasuta and C. hamulosa and developed conventional multiplex PCR method using species-specific primers for discriminating between the two species. The method amplified 455-bp and 319-bp fragments specific to D. nasuta and C. hamulosa, respectively, and did not produce them against the other chicken nematode species, Ascaridia galli, Oxyspirura mansoni, Heterakis gallinarum, Heterakis beramporia, and Heterakis indica, suggesting that the multiplex PCR is sensitive and available for species diagnosis.","subitem_description_type":"Abstract"}]},"item_16_link_13":{"attribute_name":"抄録（URL）","attribute_value_mlt":[{"subitem_link_text":"Development of conventional multiplex PCR method for discrimination between Dispharynx nasuta and Cheilospirura hamulosa (Nematoda: Acuariidae) parasitizing poultry","subitem_link_url":"https://www.jstage.jst.go.jp/article/jvms/83/2/83_20-0664/_article/"}]},"item_16_publisher_14":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Society of Veterinary Science"}]},"item_16_relation_26":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1292/jvms.20-0664","subitem_relation_type_select":"DOI"}}]},"item_16_rights_18":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"©2021 The Japanese Society of Veterinary Science"}]},"item_16_rights_19":{"attribute_name":"権利URI","attribute_value_mlt":[{"subitem_rights":"https://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_16_source_id_25":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA10796138","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA11510037","subitem_source_identifier_type":"NCID"}]},"item_16_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13477439","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"09167250","subitem_source_identifier_type":"ISSN"}]},"item_16_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University ,  Department of Pathogenetic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University"},{"subitem_text_value":"Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University ,  Department of Pathogenetic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University"},{"subitem_text_value":"Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University , Department of Pathogenetic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University"},{"subitem_text_value":"Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University , Department of Pathogenetic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University"}]},"item_16_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"BISWAS, Peru Gopal"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OHARI, Yuma"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MOHANTA, Uday Kumar"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ITAGAKI, Tadashi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-08-17"}],"displaytype":"detail","filename":"jvms-v83n2p226-229.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"jvms-v83n2p226-229","url":"https://iwate-u.repo.nii.ac.jp/record/15760/files/jvms-v83n2p226-229.pdf"},"version_id":"a5e0971b-7491-4c29-8354-f1d9a126f71c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cheilospirura hamulosa","subitem_subject_scheme":"Other"},{"subitem_subject":"Dispharynx nasuta","subitem_subject_scheme":"Other"},{"subitem_subject":"multiplex PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"nuclear ribosomal 18S-ITS1-5.8S-ITS2-28S DNA","subitem_subject_scheme":"Other"},{"subitem_subject":"poultry","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development of conventional multiplex pcr method for discrimination between dispharynx nasuta and cheilospirura hamulosa ( Nematoda : Acuariidae ) parasitizing poultry","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of conventional multiplex pcr method for discrimination between dispharynx nasuta and cheilospirura hamulosa ( Nematoda : Acuariidae ) parasitizing poultry"}]},"item_type_id":"16","owner":"19","path":["1510"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-08-17"},"publish_date":"2022-08-17","publish_status":"0","recid":"15760","relation_version_is_last":true,"title":["Development of conventional multiplex pcr method for discrimination between dispharynx nasuta and cheilospirura hamulosa ( Nematoda : Acuariidae ) parasitizing poultry"],"weko_creator_id":"19","weko_shared_id":-1},"updated":"2023-05-15T12:51:59.238621+00:00"}