@article{oai:iwate-u.repo.nii.ac.jp:00015772,
 author = {OCHIAI, Chihiro and MIYAUCHI, Sonoko and KUDO, Yuta and NARUKE, Yuta and YONEYAMA, Syuji and TOMITA, Keisuke and DONGZE, Leng and CHIBA, Yusuke and HIRATA, To-Ichi and ICHIJO, Toshihiro and NAGAI, Kazuya and KOBAYASHI, Sota and YAMADA, Shinji and HIKONO, Hirokazu and MURAKMI, Kenji},
 issue = {9},
 journal = {PLoS ONE},
 month = {Sep},
 note = {Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL), a malignant B cell lymphoma. However, the mechanisms of BLV-associated lymphomagenesis remain poorly understood. Here, after deep sequencing, we performed comparative analyses of B cell microRNAs (miRNAs) in cattle infected with BLV and those without BLV. In BLV-infected cattle, BLV-derived miRNAs (blv-miRNAs) accounted for 38% of all miRNAs in B cells. Four of these blv-miRNAs (blv-miR-B1-5p, blv-miR-B2-5p, blv-miR-B4-3p, and blv-miR-B5-5p) had highly significant positive correlations with BLV proviral load (PVL). The read counts of 90 host-derived miRNAs (bta-miRNAs) were significantly down-regulated in BLV-infected cattle compared to those in uninfected cattle. Only bta-miR-375 had a positive correlation with PVL in BLV-infected cattle and was highly expressed in the B cell lymphoma tissue of EBL cattle. There were a few bta-miRNAs that correlated with BLV tax/rex gene expression; however, BLV AS1 expression had a significant negative correlation with many of the down-regulated bta-miRNAs that are important for tumor development and/or tumor suppression. These results suggest that BLV promotes lymphomagenesis via AS1 and blv-miRNAs, rather than tax/rex, by down-regulating the expression of bta-miRNAs that have a tumor-suppressing function, and this downregulation is linked to increased PVL.},
 title = {Characterization of microRNA expression in B cells derived from Japanese black cattle naturally infected with bovine leukemia virus by deep sequencing},
 volume = {16},
 year = {2021}
}