| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-09-12 |
| タイトル |
|
|
タイトル |
Calpain-1 C2L domain peptide protects mouse hippocampus-derived neuronal HT22 cells against glutamate-induced oxytosis |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Mitochondrial calpain-1 |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Oxytosis |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Cell penetrating peptide |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Hippocampal HT22 cells |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Neurodegeneration |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
SUGAWARA, Mayu
ABE, Takumi
KASAI, Shuya
ITOH, Ken
OZAKI, Taku
|
| 著者(機関) |
|
|
値 |
Laboratory of Cell Biochemistry, Department of Biological Science, Graduate School of Science and Engineering, Iwate University |
| 著者(機関) |
|
|
値 |
Laboratory of Cell Biochemistry, Department of Biological Science, Graduate School of Science and Engineering, Iwate University |
| 著者(機関) |
|
|
値 |
Department of Stress Response Science, Center for Advanced Medical Research, Hirosaki University Graduate School of Medicine |
| 著者(機関) |
|
|
値 |
Department of Stress Response Science, Center for Advanced Medical Research, Hirosaki University Graduate School of Medicine |
| 著者(機関) |
|
|
値 |
Laboratory of Cell Biochemistry, Department of Biological Science, Graduate School of Science and Engineering, Iwate University |
| 登録日 |
|
|
日付 |
2022-09-12 |
| 書誌情報 |
Biochemistry and Biophysics Reports
巻 27,
p. 101101,
発行日 2021-08-12
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
24055808 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Calpains are Ca2+-dependent cysteine proteases; their aberrant activation is associated with several neurodegenerative diseases. The μ-calpain catalytic subunit, calpain-1, is located in the cytoplasm as well as in the mitochondria. Mitochondrial calpain-1 cleaves apoptosis-inducing factor (AIF), leading to apoptotic cell death. We have previously reported that short peptides of calpain-1 C2-like domain conjugated with cell penetrating peptide HIV-Tat (Tat-μCL) selectively inhibit mitochondrial calpain-1 and effectively prevent neurodegenerative diseases of the eye. In this study, we determined whether mitochondrial calpain-1 mediates oxytosis (oxidative glutamate toxicity) in hippocampal HT22 cells using Tat-μCL and newly generated polyhistidine-conjugated μCL peptide and compared their efficacies in preventing oxytosis. TUNEL assay and single strand DNA staining revealed that both μCL peptides inhibited glutamate-induced oxytosis. Additionally, both the peptides suppressed the mitochondrial AIF translocation into the nucleus. All polyhistidine-μCL peptides (containing 4–16 histidine residues) showed higher cell permeability than Tat-μCL. Notably, tetrahistidine (H4)-μCL exerted the highest cytoprotective activity. Thus, H4-μCL may be a potential peptide drug for calpain-1-mediated neurodegenerative diseases such as Alzheimer's disease. |
| 抄録（URL） |
|
|
表示名 |
Calpain-1 C2L domain peptide protects mouse hippocampus-derived neuronal HT22 cells against glutamate-induced oxytosis |
|
URL |
https://www.sciencedirect.com/science/article/pii/S2405580821001965 |
| 出版者 |
|
|
出版者 |
Elsevier B.V. |
| 権利 |
|
|
権利情報 |
© 2021 The Authors. |
| 権利URI |
|
|
権利情報 |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
info:doi/10.1016/j.bbrep.2021.101101 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
bbr-v27p101101 (4.8 MB)
