{"created":"2023-05-15T12:10:08.779494+00:00","id":15796,"links":{},"metadata":{"_buckets":{"deposit":"73bfdeff-4b85-4dfd-b8ef-b404763777a3"},"_deposit":{"created_by":19,"id":"15796","owners":[19],"pid":{"revision_id":0,"type":"depid","value":"15796"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00015796","sets":["1515:1519"]},"author_link":["95187","95185","95189","95193","95190","95186","95184","95191","95188","95192"],"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-10-14","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"64","bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"npj Regenerative Medicine"}]}]},"item_16_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2022-09-20"}]},"item_16_description_11":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Optogenetic gene-mediated therapy for restoring vision is thought to be a useful treatment for blind patients. However, light sensitivity achieved using this gene therapy is inferior to that of daylight vision. To increase light sensitivity, we designed three mutants using a bioinformatics approach. Nucleotide sequences encoding two sites in the extracellular loops (ex1, ex3) of mVChR1 close to simulated ion-conducting pathways were replaced by homologous amino acid-encoding sequences of ChR1 or ChR2. The light sensitivity of ex3mV1 was higher than that of mVChR1 at 405–617 nm. Visual responses were restored in Royal College of Surgeons rats with genetically degenerating photoreceptor cells transfected with ex3mV1Co, wherein transmembrane of sixth (TM6) in ex3mV1 was additionally replaced with the corresponding domain of CoChR; these rats responded to light in the order of μW/mm2. Thus, ex3mV1Co might be useful for the restoration of advanced visual function.","subitem_description_type":"Abstract"}]},"item_16_link_13":{"attribute_name":"抄録（URL）","attribute_value_mlt":[{"subitem_link_text":"Development of an optogenetic gene sensitive to daylight and its implications in vision restoration","subitem_link_url":"https://www.nature.com/articles/s41536-021-00177-5"}]},"item_16_publisher_14":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature Research"}]},"item_16_relation_26":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1038/s41536-021-00177-5","subitem_relation_type_select":"DOI"}}]},"item_16_rights_18":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2021"}]},"item_16_rights_19":{"attribute_name":"権利URI","attribute_value_mlt":[{"subitem_rights":"https://creativecommons.org/licenses/by/4.0/"}]},"item_16_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"20573995","subitem_source_identifier_type":"ISSN"}]},"item_16_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering"},{"subitem_text_value":"Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering"},{"subitem_text_value":"Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering"},{"subitem_text_value":"Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering"},{"subitem_text_value":"Department of Nutrition, Kyushu Nutrition Welfare University"},{"subitem_text_value":"Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering"},{"subitem_text_value":"Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering"},{"subitem_text_value":"Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering"},{"subitem_text_value":"Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering"},{"subitem_text_value":"Graduate Course in Biological Sciences, Iwate University Division of Science and Engineering , Clinical Research, Innovation and Education Center, Tohoku University Hospital"}]},"item_16_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"WATANABE, Yoshito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SUGANO, Eriko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TABATA, Kitako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HATAKEYAMA, Akito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SAKAJIRI, Tetsuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"FUKUDA, Tomokazu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OZAKI, Taku"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SUZUKI, Tomoya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SAYAMA, Tatsuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TOMITA, Hiroshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-09-20"}],"displaytype":"detail","filename":"npjrm-v6p64.pdf","filesize":[{"value":"3.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"npjrm-v6p64","url":"https://iwate-u.repo.nii.ac.jp/record/15796/files/npjrm-v6p64.pdf"},"version_id":"21fdcdfb-bbfc-47e3-9611-b064ecb82196"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development of an optogenetic gene sensitive to daylight and its implications in vision restoration","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of an optogenetic gene sensitive to daylight and its implications in vision restoration"}]},"item_type_id":"16","owner":"19","path":["1519"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-09-20"},"publish_date":"2022-09-20","publish_status":"0","recid":"15796","relation_version_is_last":true,"title":["Development of an optogenetic gene sensitive to daylight and its implications in vision restoration"],"weko_creator_id":"19","weko_shared_id":-1},"updated":"2023-05-15T12:50:39.790436+00:00"}