{"created":"2025-07-24T01:37:40.699782+00:00","id":2000808,"links":{},"metadata":{"_buckets":{"deposit":"dda632bb-85b8-4bbb-8f70-2805d4f3f85b"},"_deposit":{"created_by":19,"id":"2000808","owner":"19","owners":[19],"pid":{"revision_id":0,"type":"depid","value":"2000808"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:02000808","sets":["1523:1670"]},"author_link":[],"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2025-03-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicNumberOfPages":"4","bibliographicPageEnd":"90","bibliographicPageStart":"87","bibliographicVolumeNumber":"39","bibliographic_titles":[{"bibliographic_title":"日本科学教育学会研究会研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"JSSE Research Report","bibliographic_titleLang":"en"}]}]},"item_16_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2025-07-24","subitem_date_issued_type":"Created"}]},"item_16_description_11":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"真核生物の細胞内には細胞小器官があり，そのうち核，ミトコンドリア，及び葉緑体はそれぞれのDNAを有す．これについては高等学校の生物の授業で学ぶが，知識の定着していない学習者が少なからず見受けられ，対策が必要であると思われる．そこで私たちは，PCR実験を通して知識の定着を図るために，市販の乾燥ワカメを出発材料として，核DNAの一部（195bp），ミトコンドリアDNAの一部（317bp），及び葉緑体DNAの一部（401bp）の計3領域を1つの反応液で増幅するマルチプレックスPCRを検討した．その結果，アガロースゲル電気泳動にて想定通りの3本の増幅産物が明瞭にみとめられる実験条件を見出した．実験結果の再現性を確認した後に，検討結果を整理して実験プロトコルを作成し，これに従うことでPCR未経験者も良好な結果が得られることを確認した．今後，このプロトコルを適用したPCR体験学習を開催し，高校生に学習する場を提供する予定である．","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_16_publisher_14":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"一般社団法人日本科学教育学会","subitem_publisher_language":"ja"},{"subitem_publisher":"Japan Society for Science 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