{"created":"2023-05-15T12:04:24.061553+00:00","id":8943,"links":{},"metadata":{"_buckets":{"deposit":"4bfe271e-f144-40f1-9036-87e07c9c2f59"},"_deposit":{"created_by":3,"id":"8943","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"8943"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00008943","sets":["1504:1505"]},"author_link":["70928","70927"],"item_28_alternative_title_23":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Study on acid tolerance of highbush blueberry (Vaccinium corybosum L.) by in vitro tissue culture."}]},"item_28_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-03-01","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{}]}]},"item_28_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2011-11-22"}]},"item_28_description_11":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"科学研究費補助金[一般研究C](課題番号06660024)研究成果報告書","subitem_description_type":"Abstract"}]},"item_28_description_12":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"ブルーベリーは好酸性植物として知られるが,その耐酸性機構は明らかでない。本研究は,in vitro培養植物体を中心に,pHおよび酸性土壌で活性化するアルミニウム(A1)の影響について検討した。得られた結果は以下の通りである。\n\n1.ほ場におけるブルーベリー樹のA1含量は茎葉部よりも根部で多く,地下部に集積されていた。ポリフェノール含量は,逆に茎葉中に多くA1の結合体とは考え難かった。\n\n2.培養シュートの成長に対する培地pHの影響は,2継代にわたり培養したが,オートクレープ前pHが3.5から6.5の範囲では,pH6.5以外のいずれの培地でも成長量に大差なかった。ただし,pH6.5の培地では生育が抑制された。一方,培地中のA1は添加濃度が高くなるにつれて生育を抑制し,シュート中のA1含量も高まった。このことは,根がないと茎葉中にA1が吸収され生育を抑制することを示し,根が地上部へのA1の移動を抑制していることを示唆している。\n\n3.発根培養シュートの根端細胞の活性に対するpHおよびA1濃度の影響をFDA-PI染色により検討した結果,pH3.5の酸性緩衝液に6時間浸漬しても細胞活性は高かった。しかし,pH6.0でも同様に活性は高く,シュートのみの生育反応とは異なった。A1濃度に対する反応は,1mM処理では根端細胞は健全に生きており,耐性がみられたものの,10mMでは全て損傷していた。\n\n培養細胞の成長に対しては,pH3.5の倍地では生育が抑制され,細胞レベルでは耐酸性は見られなかった。A1に対して100μMでは生育が抑制されたが,10μM以下では正常な成長を示したことから,A1耐性の濃度は10〜100μMの間にあると思われた。","subitem_description_type":"Other"},{"subitem_description":"The responce of highbush Blueberry to pH and added Aluminium (Al) was studied in orchard grown and in vitro cultured plant. (1) Al concentration of blueberry grown in orchard was higher in the fine roots than in the new leaves and stems. (2) The rate of shoot growth in vitro cultured was no significant difference among the pH variation from 3.5 to 6.5 culture medium, except pH 6.5 medium. The pH 6.5 medium resulted in growth reduction. (3) Al in the medium (pH 5.0) inhibited the growth of shoot with increase of Al concentration, and increased Al concentration in the shoot. (4) The activity of root tip cells of the rooted shoot in vitro cultured, was high with soakage in the pH 3.5 buffer solution at 6 hr, by stainning with fluorescein diacetate (FDA) and Propidium iodide (PI). While the root tip cells were alive and high activity in pH4.5 buffer solution added 1 mM Al, but all of these were dead in 10 mM Al. (5) The medium regulated pH 3.5 reduced the growth of cells suspension cultured, then no tolerance of pH was observed in the free cells. While, the cells tolerated Al additions up to 10muM with normal growth, but 100muM Al inhibited the growth of these cells. So it was indicated that the Al tolerance in the suspension cells was between 10 and 100 muM Al.","subitem_description_type":"Other"}]},"item_28_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"SUZUKI, Akira"}]}]},"item_28_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"岩手大学農学部"}]},"item_28_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"壽松木, 章"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-11-14"}],"displaytype":"detail","filename":"kaken06660024.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"kaken06660024.pdf","url":"https://iwate-u.repo.nii.ac.jp/record/8943/files/kaken06660024.pdf"},"version_id":"ff3e3658-c11c-454c-8c14-334753be12e0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Acid tolerance","subitem_subject_scheme":"Other"},{"subitem_subject":"Aluminium","subitem_subject_scheme":"Other"},{"subitem_subject":"Blueberry","subitem_subject_scheme":"Other"},{"subitem_subject":"in vitro culture","subitem_subject_scheme":"Other"},{"subitem_subject":"suspension","subitem_subject_scheme":"Other"},{"subitem_subject":"アルミニウム","subitem_subject_scheme":"Other"},{"subitem_subject":"ブルーベリー","subitem_subject_scheme":"Other"},{"subitem_subject":"ポリフェノール","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞培養","subitem_subject_scheme":"Other"},{"subitem_subject":"組織培養","subitem_subject_scheme":"Other"},{"subitem_subject":"耐酸性","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"組織培養によるブルーベリーの耐酸性機構の解明","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"組織培養によるブルーベリーの耐酸性機構の解明"}]},"item_type_id":"28","owner":"3","path":["1505"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-11-22"},"publish_date":"2011-11-22","publish_status":"0","recid":"8943","relation_version_is_last":true,"title":["組織培養によるブルーベリーの耐酸性機構の解明"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-16T11:21:42.507875+00:00"}