{"created":"2023-05-15T12:04:24.149463+00:00","id":8945,"links":{},"metadata":{"_buckets":{"deposit":"96a6bcac-5334-4a78-a8f0-c2ff9ce62872"},"_deposit":{"created_by":3,"id":"8945","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"8945"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00008945","sets":["1504:1505"]},"author_link":["70931","70932"],"item_28_alternative_title_23":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Factor of suppression of tissue protein degradation after feeding and its mechanism"}]},"item_28_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000-05-01","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{}]}]},"item_28_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2011-12-16"}]},"item_28_description_11":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"科学研究費補助金[基盤研究(C)(2)](課題番号09660126)研究成果報告書","subitem_description_type":"Abstract"},{"subitem_description":"巻末の学術雑誌掲載論文は削除しております．","subitem_description_type":"Abstract"}]},"item_28_description_12":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Sprague-Dawley系雄ラットを1日6時間だけ飼料を摂取するように慣らさせた後に、20%カゼイン食または無タンパク質食を1時間だけ摂取させた。血清3-メチルヒスチジン(MeHis)濃度と後肢筋からのMeHis放出速度(筋原線維タンパク質の分解速度)はカゼイン食では摂食3-6時間後に有意に減少したが、無タンパク質食では変化がなかった。タンパク質含量を増加させて分解速度を測定したところ、摂取タンパク質の濃度依存的に分解が抑制された。また、摂取タンパク質量を一定にしてエネルギー量を減らしても分解の抑制が認められた。これらは摂取タンパク質が摂取直後の分解を調節していることを示している。ラットに窒素源としてロイシンのみを含む食餌を与えたときも同様の分解の変化が認められた。さらに、ロイシン懸濁液を直接胃内投与すると、筋肉からのMeHis放出速度は直ちに減少したことから、食餌タンパク質による分解抑制作用には食餌タンパク質中のロイシンが関係していることが明らかとなった。\n\n摂食後の筋原線維タンパク質の分解抑制に関与する分解系を解析した。リソソームによる分解系とカルシウムにより活性化される分解系を阻害すると摂食後の分解抑制が認められなくなったが、ATP-依存性の分解系を阻害しても分解抑制の傾向には変化がなかった。したがって、摂食後の分解抑制にはリソソームあるいはカルパインが関与していることが示唆された。 摂食後のタンパク質合成活性をシグナル伝達系から解析した。摂食後すぐにp70S6kのリン酸化が認められ、無タンパク質食では認められなかった。したがって、タンパク質(ロイシン)は摂食後ただちにp70S6kのリン酸化などを通じてタンパク質合成を促進し、それより遅れてカルパイン活性の変化などを介して分解を抑制することが考えられた。","subitem_description_type":"Other"},{"subitem_description":"After feeding of 20% casein diet for 1h, the rate of myofibrillar protein degradation measured by the serum concentration of 3-methyl-histidine (MeHis) and the release rate of MeHis from the isolated muscle significantly decreased, although they did not change when rats fed protein free diet. The suppression was increased with content of dietary protein content and it was not changed when feeding protein content was constant but energy was decreased. These results strongly suggest that dietary protein directly regulate myofibrillar protein degradation after feeding. Leu in dietary protein mainly affect the suppression, since these change was also seen in rats fed on Leu diet or force fed on Leu suspension.\n\nMechanism of suppression of degradation after feeding was analyzed by proteolysis inhibitors. When lysosome and calcium-dependent proteolytic systems were inhibited, the suppression was not seen, indicating that calpain may key enzyme. However, when ATP-dependent proteolytic system was inhibited, the suppression of myofibrillar protein degradation after feeding was demonstrated.\n\nPhosphorylation of p70S6 kinase which correlates with protein synthesis was immediately activated by protein and Leu feeding. Therefore, protein synthesis may be activated more rapidly than protein degradation by feeding.","subitem_description_type":"Other"}]},"item_28_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"70932","nameIdentifierScheme":"WEKO"}],"names":[{"name":"NAGASAWA, Takashi"}]}]},"item_28_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"岩手大学農学部"}]},"item_28_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"長澤, 孝志"}],"nameIdentifiers":[{"nameIdentifier":"70931","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-11-14"}],"displaytype":"detail","filename":"kaken09660126.pdf","filesize":[{"value":"3.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"kaken09660126.pdf","url":"https://iwate-u.repo.nii.ac.jp/record/8945/files/kaken09660126.pdf"},"version_id":"312e93f0-f7cb-4906-ba2e-e3f59d686683"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"3-Methylhistidine","subitem_subject_scheme":"Other"},{"subitem_subject":"Dietary protein","subitem_subject_scheme":"Other"},{"subitem_subject":"Leucine","subitem_subject_scheme":"Other"},{"subitem_subject":"Myofibrillar protein","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein degradation","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein synthesis","subitem_subject_scheme":"Other"},{"subitem_subject":"p70S6 kinase","subitem_subject_scheme":"Other"},{"subitem_subject":"3-メチルヒスチジン","subitem_subject_scheme":"Other"},{"subitem_subject":"p70S6キナーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"キビ","subitem_subject_scheme":"Other"},{"subitem_subject":"タンパク質分解","subitem_subject_scheme":"Other"},{"subitem_subject":"タンパク質合成","subitem_subject_scheme":"Other"},{"subitem_subject":"ユビキチン","subitem_subject_scheme":"Other"},{"subitem_subject":"リソソーム","subitem_subject_scheme":"Other"},{"subitem_subject":"ロイシン","subitem_subject_scheme":"Other"},{"subitem_subject":"ロイジン","subitem_subject_scheme":"Other"},{"subitem_subject":"必須アミノ酸","subitem_subject_scheme":"Other"},{"subitem_subject":"筋原線維タンパク質","subitem_subject_scheme":"Other"},{"subitem_subject":"食餌タンパク質","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"摂食直後の組織タンパク質分解抑制因子の検索と作用機構","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"摂食直後の組織タンパク質分解抑制因子の検索と作用機構"}]},"item_type_id":"28","owner":"3","path":["1505"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-12-16"},"publish_date":"2011-12-16","publish_status":"0","recid":"8945","relation_version_is_last":true,"title":["摂食直後の組織タンパク質分解抑制因子の検索と作用機構"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-16T11:21:39.664549+00:00"}