{"created":"2023-05-15T12:04:24.237211+00:00","id":8947,"links":{},"metadata":{"_buckets":{"deposit":"d461e319-b2ac-45f4-b5f7-191aa7493c3b"},"_deposit":{"created_by":3,"id":"8947","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"8947"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00008947","sets":["1504:1505"]},"author_link":["70936","70935"],"item_28_alternative_title_23":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Different response of amino acids on muscle protein synthesis and degradation under nutritional stresses"}]},"item_28_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-06-01","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{}]}]},"item_28_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2011-12-19"}]},"item_28_description_11":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"科学研究費補助金[基盤研究C](課題番号18580110)研究成果報告書","subitem_description_type":"Abstract"},{"subitem_description":"巻末の学術雑誌掲載論文は削除しております．","subitem_description_type":"Abstract"}]},"item_28_description_12":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"1.低栄養状態におけるロイシンの作用\n\nラットに無タンパク質食を1週間自由摂取させると後肢筋重量は減少したが、それに1.5%のロイシンを添加した食餌を与えると骨格筋量の減少は抑制された。このときの筋原線維タンパク質の分解速度を3-メチルヒスチジン放出速度を新たに開発した動静脈濃度差法で測定したところ、ロイシンの投与で有意に減少した。骨格筋タンパク質の合成速度は両者で変化は認められなかった。ユビキチン-プロテアソーム系の活性やユビキチンリガーゼの発現の減少はなく、オートファジーの律速となるオートファゴソーム形成の調節タンパク質であるLC3-IからLC3-IIへの変換が抑制されたことを見いだした。これは、分解の抑制がオートファージー-リソソーム系で行われていることを示唆している。\n\n2.6時間ごとのロイシンの摂取が合成と分解に及ぼす影響\n\n20%カゼイン食で飼育しているラットを18時間絶食させ、ロイシンを6時間ごとに2回67.5mg/100g体重ずつ経口投与した。3-メチルヒスチジンの放出速度から測定した分解速度は、投与1時間後から減少し6時間後まで減少が続き、2回目の投与でさらに減少した。合成の指標となるS6K1のリン酸化は投与1時間後だけ促進された。以上の結果から、低栄養というストレス時において骨格筋タンパク質の増減は、合成よりむしろ分解で調節され、それを制御することで骨格筋の萎縮抑制できることが明らかになった。\n\n3.廃用性筋萎モデルにおけるロイシンの効果\n\n10%カゼインあるいはそれにロイシンを1%添加した食餌を摂取させたラットの後肢をギプスで固定し、分解を評価した結果、固定により増加した分解をロイシンは抑制し、ユビキチンリガーゼ遺伝子発現にも影響を与えることを明らかにした。","subitem_description_type":"Other"},{"subitem_description":"1. The function of leucine under low nutritional conditions Skeletal muscle weight decreased in feeding of one week protein free diet, however the weight loss was suppressed in 1.5% leucine supplementation. Muscle protein degradation evaluated by A-V difference of 3-methylhistidine concentration was also suppressed by leucine supplemented diet. The rate of muscle protein synthesis was not changed. Although ubiquitin-proteasome expression and calpain activity were not reduced in feeding of leucine supplemented diet, LC-3II expression was decreased. These results suggested that leucine enhances muscle protein degradation rather synthesis and the degradation might be regulated by autophagy in low nutritional conditions.\n\n2. Effects of repeated leucine consumption on muscle protein synthesis and degrdation Six hours interval leucine administration to rats decreased myofibrillar protein degradation evaluated by 3-methylhistidine release from the isolated muscle. However, synthesis evaluated by phosphrylation of S6K1 did not, suggesting that repeated leucine supplementation is beneficial for muscle atrophy.\n\n3. Effects of leucine on muscle atrophy in disuse model Immobilization of hind limb increased muscle protein degradation and then resulted muscle atrophy. In this model, leucine supplemented diet attenuated muscle atrophy due to muscle protein degradation especially ubiquitin-proteasome.","subitem_description_type":"Other"}]},"item_28_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"70936","nameIdentifierScheme":"WEKO"}],"names":[{"name":"NAGASAWA, Takashi"}]}]},"item_28_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"岩手大学農学部"}]},"item_28_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"長澤, 孝志"}],"nameIdentifiers":[{"nameIdentifier":"70935","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-11-14"}],"displaytype":"detail","filename":"kaken18580110.pdf","filesize":[{"value":"4.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"kaken18580110.pdf","url":"https://iwate-u.repo.nii.ac.jp/record/8947/files/kaken18580110.pdf"},"version_id":"3e9f927c-79d5-4965-8496-fd0733f85395"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"amino acids","subitem_subject_scheme":"Other"},{"subitem_subject":"leucine","subitem_subject_scheme":"Other"},{"subitem_subject":"muscle","subitem_subject_scheme":"Other"},{"subitem_subject":"protein degradation","subitem_subject_scheme":"Other"},{"subitem_subject":"protein synthesis","subitem_subject_scheme":"Other"},{"subitem_subject":"アミノ酸","subitem_subject_scheme":"Other"},{"subitem_subject":"タンパク質分解","subitem_subject_scheme":"Other"},{"subitem_subject":"タンパク質合成","subitem_subject_scheme":"Other"},{"subitem_subject":"ロイシン","subitem_subject_scheme":"Other"},{"subitem_subject":"筋肉","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"栄養ストレスにおけるアミノ酸の筋肉タンパク質合成と分解に対する異なるレスポンス","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"栄養ストレスにおけるアミノ酸の筋肉タンパク質合成と分解に対する異なるレスポンス"}]},"item_type_id":"28","owner":"3","path":["1505"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-12-19"},"publish_date":"2011-12-19","publish_status":"0","recid":"8947","relation_version_is_last":true,"title":["栄養ストレスにおけるアミノ酸の筋肉タンパク質合成と分解に対する異なるレスポンス"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-16T11:21:37.556166+00:00"}