| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2009-02-10 |
| タイトル |
|
|
タイトル |
Molecular cloning and mapping of casein kinase 2 alpha and beta subunit genes in barley |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
barley |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
casein kinase 2 |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
cDNA cloning |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
RFLP mapping |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Kato, Kiyoaki
Kidou, Shin-ichiro
Miura, Hideho
|
| 著者(機関) |
|
|
値 |
Department of Crop Science, Obihiro University of Agriculture and Veterinary Medicine |
| 著者(機関) |
|
|
値 |
Cryobiosystem Research Center, Faculty of Agriculture,Iwate University |
| 著者(機関) |
|
|
値 |
Department of Crop Science, Obihiro University of Agriculture and Veterinary Medicine |
| 登録日 |
|
|
日付 |
2009-02-10 |
| 書誌情報 |
Genome
巻 51,
号 3,
p. 208-215,
発行日 2008-01-01
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0831-2796 |
| Abstract |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Casein kinase 2 (CK2) is a ubiquitous, highly pleiotropic, constitutively active, and messenger-independent Ser/Thr protein kinase. It is found in two different forms: the heterotetrameric CK2, composed of two alpha catalytic subunits and two beta regulatory subunits, and the monomeric CK2 alpha, consisting of the alpha catalytic subunit. In the present study, we isolated barley cDNA clones of the CK2 alpha and beta subunit genes, designated HvCK2A and HvCK2B, respectively. Chromosome assignment, using a set of wheat–barley disomic chromosome addition lines, and RFLP mapping, using two doubled haploid populations, showed that HvCK2A was duplicated on the short arm of chromosome 2H and the long arm of chromosome 5H (designated HvCK2a-2H and HvCK2a-5H, respectively), and a single copy of HvCK2B was located on the long arm of chromosome 1H (designated HvCK2b). A PCR-Southern hybridization experiment demonstrated that the HvCK2A sequence originated from the HvCK2a-5H locus, showing that at least HvCK2a-5H was expressed. The present cDNA sequences and genomic organization of the two subunits will facilitate further functional analysis of CK2 in barley. |
| 出版者 |
|
|
出版者 |
National Research Council Canada |
| 権利 |
|
|
権利情報 |
© 2008 NRC Canada |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1139/G07-092 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
genome-v51n3p208-215.pdf (812.1 kB)
