| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2009-08-25 |
| タイトル |
|
|
タイトル |
Microbial resolution of Nε-acetyl-DL-lysine with Rhodococcus sp. AIU Z-35-1 |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Nα-benzyloxycarbonyl-l-lysine |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Nα-benzyloxycarbonyl-d-lysine |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Nε-acetyl-l-lysine |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Nε-acetyl-d-lysine |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
6-Acetylamino-2-oxohexanoic acid |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Isobe, Kimiyasu
Tamauchi, Hiroshi
Nagasawa, Shouko
|
| 著者(機関) |
|
|
値 |
Department of Biological Chemistry and Food Science, Faculty of Agriculture,Iwate University |
| 登録日 |
|
|
日付 |
2009-08-25 |
| 書誌情報 |
Journal of Molecular Catalysis B: Enzymatic
巻 59,
号 1-3,
p. 47-51,
発行日 2009-07-01
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1381-1177 |
| Abstract |
|
|
内容記述タイプ |
Other |
|
内容記述 |
An enantioselective resolution of Nε-acetyl-dl-lysine was investigated using cells from Rhodococcus sp. AIU Z-35-1 cultivated with l-lysine medium. Nε-Acetyl-l-lysine was deaminated by the resting cells, and 6-acetylamino-2-oxohexanoic acid was formed, but Nε-acetyl-d-lysine was not. This reaction was optimum at pH 6.5 and 30 °C, and the highest reaction speed was obtained by cells harvested after 1 day of cultivation. When 150 mM Nε-acetyl-dl-lysine was incubated at pH 6.5 and 30 °C for 5 days with cells harvested after 1 day of cultivation, more than 98% of the Nε-acetyl-l-lysine was converted to 6-acetylamino-2-oxohexanoic acid, and Nε-acetyl-d-lysine completely remained. Thus, Nε-acetyl-d-lysine was enantioselectively produced from Nε-acetyl-dl-lysine by the cell reaction with Rhodococcus sp. AIU Z-35-1. This cell reaction was also useful for the efficient production of 6-acetylamino-2-oxohexanoic acid from Nε-acetyl-l-lysine. |
| 出版者 |
|
|
出版者 |
Elsevier B.V. |
| 権利 |
|
|
権利情報 |
Copyright © 2009 Elsevier B.V |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.molcatb.2008.12.019 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
jmcbe-v59i1-3p47-51.pdf (231.3 kB)
