@article{oai:iwate-u.repo.nii.ac.jp:00009150,
 author = {Tanaka, Daisuke and Niino, Takao and Tsuchiya, Yoshiko and Shirata, Kazuto and Uemura, Matsuo},
 issue = {2},
 journal = {Plant Genetic Resources: Characterization and Utilization},
 month = {Jan},
 note = {Hayachine-usuyukiso (Leontopodium hayachinense) is an alpine plant native to Mt Hayachine.
This unique chrysanthemum is listed as an endangered plant by the Department of Conservation, Iwate Prefecture, and as a threatened plant by the Ministry of the Environment, Japan.
We successfully cryopreserved the shoot tips from in vitro-grown L. hayachinense shoots
using a vitrification protocol. Cold-hardened shoot tips were excised and pre-cultured on a solidified Murashige–Skoog medium containing 0.3M sucrose for 1 d at 58C. The shoot tips were then treated with loading solution for 20 min at 258C, dehydrated in plant vitrification solution
2 for 120 min at 258C and immersed in liquid nitrogen. The survival rate of the vitrified
shoot tips was 63.3% after 30 d of regrowth. This protocol appears to be a promising technique for the cryopreservation of in vitro-grown shoots of this endangered plant.},
 pages = {164--166},
 title = {Cryopreservation of shoot tips of endangered Hayachine-usuyukiso (Leontopodium hayachinense (Takeda) Hara et Kitam.) using a vitrification protocol},
 volume = {6},
 year = {2008}
}