{"created":"2023-05-15T12:04:35.683990+00:00","id":9150,"links":{},"metadata":{"_buckets":{"deposit":"911e41a4-5cc2-4b43-8219-c031f1e31340"},"_deposit":{"created_by":3,"id":"9150","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"9150"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00009150","sets":["1504:1510"]},"author_link":["71947","71946","71945","71949","71948"],"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"166","bibliographicPageStart":"164","bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"Plant Genetic Resources: Characterization and Utilization"}]}]},"item_16_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2010-01-13"}]},"item_16_description_12":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Hayachine-usuyukiso (Leontopodium hayachinense) is an alpine plant native to Mt Hayachine.\nThis unique chrysanthemum is listed as an endangered plant by the Department of Conservation, Iwate Prefecture, and as a threatened plant by the Ministry of the Environment, Japan.\nWe successfully cryopreserved the shoot tips from in vitro-grown L. hayachinense shoots\nusing a vitrification protocol. Cold-hardened shoot tips were excised and pre-cultured on a solidified Murashige–Skoog medium containing 0.3M sucrose for 1 d at 58C. The shoot tips were then treated with loading solution for 20 min at 258C, dehydrated in plant vitrification solution\n2 for 120 min at 258C and immersed in liquid nitrogen. The survival rate of the vitrified\nshoot tips was 63.3% after 30 d of regrowth. This protocol appears to be a promising technique for the cryopreservation of in vitro-grown shoots of this endangered plant.","subitem_description_type":"Other"}]},"item_16_publisher_14":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Cambridge University Press"}]},"item_16_relation_26":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1017/S1479262108993175","subitem_relation_type_select":"DOI"}}]},"item_16_rights_18":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © NIAB 2008"}]},"item_16_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1479-2621","subitem_source_identifier_type":"ISSN"}]},"item_16_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"Genebank, National Institute of Agrobiological Sciences"},{"subitem_text_value":"Genebank, National Institute of Agrobiological Sciences"},{"subitem_text_value":"Graduate School of Agriculture, Iwate University"},{"subitem_text_value":"Genebank, National Institute of Agrobiological Sciences"},{"subitem_text_value":"Graduate School of Agriculture, Iwate University"}]},"item_16_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tanaka, Daisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Niino, Takao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsuchiya, Yoshiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shirata, Kazuto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Uemura, Matsuo"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-11-14"}],"displaytype":"detail","filename":"pgrcu-v6n2p164-166.pdf","filesize":[{"value":"496.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"pgrcu-v6n2p164-166.pdf","url":"https://iwate-u.repo.nii.ac.jp/record/9150/files/pgrcu-v6n2p164-166.pdf"},"version_id":"999d47e0-2163-4757-8446-d680eb4882eb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cryopreservation","subitem_subject_scheme":"Other"},{"subitem_subject":"endangered species","subitem_subject_scheme":"Other"},{"subitem_subject":"Leontopodium hayachinense (Takeda) Hara et Kitam","subitem_subject_scheme":"Other"},{"subitem_subject":"tissue culture; vitrification","subitem_subject_scheme":"Other"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cryopreservation of shoot tips of endangered Hayachine-usuyukiso (Leontopodium hayachinense (Takeda) Hara et Kitam.) using a vitrification protocol","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cryopreservation of shoot tips of endangered Hayachine-usuyukiso (Leontopodium hayachinense (Takeda) Hara et Kitam.) using a vitrification protocol"}]},"item_type_id":"16","owner":"3","path":["1510"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-01-13"},"publish_date":"2010-01-13","publish_status":"0","recid":"9150","relation_version_is_last":true,"title":["Cryopreservation of shoot tips of endangered Hayachine-usuyukiso (Leontopodium hayachinense (Takeda) Hara et Kitam.) using a vitrification protocol"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-16T11:13:26.299096+00:00"}