| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2010-02-02 |
| タイトル |
|
|
タイトル |
Establishment of New Embryonic Stem (ES) Cell Lines from C57BL/6 Strain Mice by Whole Blastocyst Culture Method |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Embryonic stem (ES) cell lines |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
C57BL/6 strain mouse |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Whole blastocyst culture method |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Chimera |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Hermaphrodite |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Osonoi, Makoto
Kim, Sang-Yong
TAKAHASHI, Jutaro
Yasuda, Yasuhisa
|
| 著者(機関) |
|
|
値 |
Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Iwate University |
| 登録日 |
|
|
日付 |
2010-02-02 |
| 書誌情報 |
Journal of Mammalian Ova Research
巻 13,
号 2,
p. 91-98,
発行日 1996-01-01
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1341-7738 |
| Abstract |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Two embryonic stem (ES) cell lines (OKB6-I and -II) derived from C57BL/6 strain mice were established by the whole blastocyst culture method, and the net isolation ratio was 3.9% (2/51). They had pluripotency and normal karyotype, and were positive for alkaline phosphatase activity. It was indicated that OKB6-I was a female cell line, and OKB6-II was a male, judged by the size and morphology of their chromosomes. By coculture with ICR embryos, a chimeric mouse and a hermaphrodite were generated from OKB6-II, but no chimera was generated from OKB6-I. OKB6-II⇔ICR chimera generated 3 albino type viable offspring, when mated with ICR strain mouse. OKB6-II⇔ICR hermaphrodite was sterile, and had a contralateral ovary and testis-like structure. |
| 出版者 |
|
|
出版者 |
Japanese Society of Mammalian Ova Research |
| 権利 |
|
|
権利情報 |
Copyright (c) 1996 by Japanese Society of Mammalian Ova Research |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
jmor-v13n2p91-98.pdf (1.9 MB)
