| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2010-02-02 |
| タイトル |
|
|
タイトル |
Expression of pMGN(-4k)LacZ-neo Gene Introduced into Embryonic Stem Cells In Vitro |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Embryonic stem (ES) cells |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Gene expression |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Myogenin gene regulatory region |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
LacZ reporter gene |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Embryoid bodies |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Osonoi, Makoto
Mimaki, Yuka
Ito, Kazue
Takahashi, Jutaro
Yasuda, Yasuhisa
|
| 著者(機関) |
|
|
値 |
Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Iwate University |
| 登録日 |
|
|
日付 |
2010-02-02 |
| 書誌情報 |
Journal of Mammalian Ova Research
巻 16,
号 1,
p. 31-36,
発行日 1999-01-01
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1341-7738 |
| Abstract |
|
|
内容記述タイプ |
Other |
|
内容記述 |
We attempted to produce a muscle-specific cell marker, which would be useful for examining myogenesis, by introducing pMGN(-4k)LacZ-neo gene into ESD3 cells, and expression of the transgene was analyzed in embryoid bodies. The gene expression analysis of embryoid bodies indicated that transfected cells did not express LacZ gene in an undifferentiated state and that the number of cells with LacZ expression increased progressively. In DMSO treated embryoid bodies, the number of cells expressing LacZ gene tended to increase more rapidly than that of untreated ones. The results suggested that the DMSO may induce mesodermal differentiation of ES cells and that the sublines obtained might be useful as muscle-specific cell markers since they might express lacZ gene at the time of myogenin expression. |
| 出版者 |
|
|
出版者 |
Japanese Society of Mammalian Ova Research |
| 権利 |
|
|
権利情報 |
Copyright (c) 1999 by Japanese Society of Mammalian Ova Research |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
jmor-v16n1p31-36.pdf (211.0 kB)
