@article{oai:iwate-u.repo.nii.ac.jp:00009192,
 author = {壽松木, 章 and 三浦, 哲人 and 青葉, 幸二},
 issue = {5},
 journal = {Journal of the Japanese Society for Horticultural Science},
 month = {Sep},
 note = {The viable root apices of highbush blueberry and apple rootstock were observed by staining with fluorescein diacetate (FDA) and propidium iodide (PI) to assess their pH and aluminum (Al) tolerance. In the pH treatment, roots were dipped in phosphate buffer solution ranging in pH from 3.5 to 6.0 for 2 to 6 hours. About 15 to 40 % of the blueberry root epidermis in all treatments except pH 6.0 for 2 hours were damaged, but the rest of the roots remained healthy. The apple rootstock 'Maruba kaido' suffered more damage at pH 3.5 than did the blueberry. In the Al treatment, blueberry roots dipped in an acetate buffer solution without Al, the root tip or the whole root was damaged ; but roots treated with 1 mM Al retained its viability similar to the untreated root ; almost all roots treated with 10 mM Al were injured. 'Maruba kaido' exhibited a tolerance to 1 mM Al. Observation of root tissue by staining with FDA and PI is a simple technique that allows visible differentiation between viable and injured cells. Furthermore, because it is a nondestructive method, it is very useful for determining root viability. ハイブッシュブルーベリー'バークレイ'およびリンゴ台木'マルバカイドウ'のin vitro培養シュートから発生させた根のpHおよびアルミニウム耐性を, フルオロセイン2酢酸(FDA)-ヨウ化プロピジウム(PI)の2重染色法で観察した.pH3.5から6.0のリン酸緩衝液で2∿6時間浸漬処理したpH処理の結果, ブルーベリー根についてはpH6.0の2時間処理以外の処理では約15∿40%の根が表皮細胞に障害を受けたが, 半数以上の根は活性を維持した.マルバカイドウ根ではpH3.5処理で障害を受けた根が多かった.Al処理において, ブルーベリー根はAl無添加処理(酢酸緩衝液のみ)で根端部または根全体に障害を受けていた.しかし, 1 mM Al処理では処理前の根と同程度の活性を示した.一方, 10 mM Al処理ではすべての根が全体に障害を受けた.マルバカイドウは1 mM Alに対して活性を示した.FDA-PI法による根細胞の観察は, 非破壊的に活性部位と障害部位を識別できることから, 根活性の診断に有効な手法であることが認められた.},
 pages = {932--936},
 title = {Observation on the Viability of Root Apex Cells Treated with Different pH and Aluminum in Highbush Blueberry (Vaccinium corymbosum L.) and Maruba kaido (Malus prunifolia Borkh. var. Ringo) Cultured in vitro},
 volume = {68},
 year = {1999}
}