{"created":"2023-05-15T12:04:38.238295+00:00","id":9196,"links":{},"metadata":{"_buckets":{"deposit":"01345a3a-fd3a-4292-beff-d8e088e7f71f"},"_deposit":{"created_by":3,"id":"9196","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"9196"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00009196","sets":["1504:1510"]},"author_link":["72212","72210","72213","72208","72209","72207","72214","72211"],"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-04-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"736","bibliographicPageStart":"733","bibliographicVolumeNumber":"394","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications"}]}]},"item_16_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2010-10-05"}]},"item_16_description_12":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"A complete reconstitution system for membrane integration of the simplest protein was developed by means of defined factors. A mutant version of Pf3 coat protein, 3L-Pf3 coat, requires neither signal recognition particle/Sec factors nor a membrane potential for its integration into the cytoplasmic membrane of Escherichia coli. Although 3L-Pf3 coat is spontaneously integrated into liposomes composed of phospholipids, diacylglycerol completely blocks such spontaneous integrations at a physiological level. Under the conditions where spontaneous integration does not occur, 3L-Pf3 coat integration was absolutely dependent on a novel integration-stimulating factor. Combination of the PURE system, an in vitro translation system composed of the purified factors involved in translation in E. coli, with liposomes containing the highly purified integration-stimulating factor revealed multiple cycles of 3L-Pf3 coat integration, achieving the complete reconstitution of membrane integration. Based on the function of the factor, we propose that the factor is named MPIase (Membrane Protein Integrase).","subitem_description_type":"Other"}]},"item_16_publisher_14":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Inc."}]},"item_16_relation_26":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bbrc.2010.03.061","subitem_relation_type_select":"DOI"}}]},"item_16_rights_18":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(c) 2010 Elsevier Inc."}]},"item_16_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"ISSN"}]},"item_16_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"Institute of Molecular and Cellular Biosciences, The University of Tokyo,\n\n(Present address)Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University"},{"subitem_text_value":"Suntory Institute for Bioorganic Research"},{"subitem_text_value":"Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo"},{"subitem_text_value":"Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo"},{"subitem_text_value":"Suntory Institute for Bioorganic Research"},{"subitem_text_value":"Suntory Institute for Bioorganic Research"},{"subitem_text_value":"Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo"},{"subitem_text_value":"Institute of Molecular and Cellular Biosciences, The University of Tokyo"}]},"item_16_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nishiyama, Ken-ichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Maeda, Masahide"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Abe, Masato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kanamori, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimamoto, Keiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kusumoto, Shoichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ueda, Takuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tokuda, Hajime"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-11-14"}],"displaytype":"detail","filename":"bbrc-v394n3p733-736.pdf","filesize":[{"value":"2.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bbrc-v394n3p733-736.pdf","url":"https://iwate-u.repo.nii.ac.jp/record/9196/files/bbrc-v394n3p733-736.pdf"},"version_id":"d3ad1167-809e-4b13-9c25-4b1cafc3412e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Diacylglycerol","subitem_subject_scheme":"Other"},{"subitem_subject":"Integration-stimulating factor","subitem_subject_scheme":"Other"},{"subitem_subject":"Liposomes","subitem_subject_scheme":"Other"},{"subitem_subject":"Membrane protein integration","subitem_subject_scheme":"Other"},{"subitem_subject":"MPIase","subitem_subject_scheme":"Other"},{"subitem_subject":"PURE system","subitem_subject_scheme":"Other"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A novel complete reconstitution system for membrane integration of the simplest membrane protein","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A novel complete reconstitution system for membrane integration of the simplest membrane protein"}]},"item_type_id":"16","owner":"3","path":["1510"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-10-05"},"publish_date":"2010-10-05","publish_status":"0","recid":"9196","relation_version_is_last":true,"title":["A novel complete reconstitution system for membrane integration of the simplest membrane protein"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-16T11:11:25.220680+00:00"}