| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2010-10-07 |
| タイトル |
|
|
タイトル |
Simple and Rapid Method for the Selection of Individual Rapeseed Plants Low in Glucosinolates |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Brassica napus |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
glucosinolates |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
glucose release |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
cotyledon |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
selection |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Ishida, Masahiko
Takahata, Yoshihito
Kaizuma, Norihiko
|
| 著者(機関) |
|
|
値 |
National Agricultural Research Center for Tohoku Region, (Present address) National Institute of Vegetable and Tea Science |
| 著者(機関) |
|
|
値 |
Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
Faculty of Agriculture, Iwate University |
| 登録日 |
|
|
日付 |
2010-10-07 |
| 書誌情報 |
Breeding science
巻 53,
号 4,
p. 291-296,
発行日 2003-12-01
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1344-7610 |
| Abstract |
|
|
内容記述タイプ |
Other |
|
内容記述 |
A simple and rapid colorimetric method for the selection of individual rapeseed plants with low glucosinolate contents was developed using one of a pair of germinated cotyledons of a single seedling and a glucose-measuring method employed in clinical tests (CII-Test method). The CII-Test method can be used to estimate the amount of glucose released by the hydrolysis of glucosinolates extracted from a germinated cotyledon. In the present experiment, HPLC analysis showed that the total glucosinolate content of germinated cotyledons was not significantly different from that of seeds three days after seed sowing. When the glucosinolate content of the seeds and/or cotyledons was estimated by the glucose-measuring CII-Test method and HPLC method, high positive correlations were recognized between both types of measurements. The CII-Test method exhibits some clear advantages in that 1) the glucosinolate content can be estimated in an individual plant, and 2)150-500 samples can be estimated by one person in one day. These advantages indicate that the glucose measuring CII-Test method is suitable for efficient and accurate selection of rapeseed plants with low glucosinolate contents in the F_2 generation. |
| 出版者 |
|
|
出版者 |
Japanese Society of Breeding |
| 権利 |
|
|
権利情報 |
Japanese Society of Breeding |
| 権利 |
|
|
権利情報 |
本文データは学協会の許諾に基づきCiNiiから複製したものである |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 異版である |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
http://ci.nii.ac.jp/naid/110001803249 |
bs-v53n4p291-296.pdf (622.0 kB)
