| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2010-10-07 |
| タイトル |
|
|
タイトル |
Identification of genes related to root shape in radish (Raphanus sativus) using suppression subtractive hybridization |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
radish (Raphanus sativus) |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
root thickness or shape |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
ESTs |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
suppression subtractive hybridization |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
real-time RT-PCR |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Zaki, Haitham E.M.
Yokoi, Shuji
Takahata, Yoshihito
|
| 著者(機関) |
|
|
値 |
Faculty of Agriculture, Iwate University |
| 登録日 |
|
|
日付 |
2010-10-07 |
| 書誌情報 |
Breeding Science
巻 60,
号 2,
p. 130-138,
発行日 2010-01-01
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1344-7610 |
| Abstract |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Radish is an important vegetable, and displays a wide variation in root shape. To understand the mechanisms controlling development and/or inheritance of different root shapes, we attempted to identify the genes expressed differentially in roots of two cultivars with different root shapes; long and thick type (Lt) cv. Taibyousoubutori and skinny type (St) cv. Kosena. Morphological studies revealed that significant differences in thickness of roots began at 4 weeks after sowing. By suppression subtractive hybridization using roots at this stage, 140 and 70 non-redundant ESTs were identified from subtraction of St (tester) × Lt (driver) and reverse subtraction, respectively. Of these ESTs, 102 and 52 showed high similarity to previously identified genes, and the remaining 38 and 18 showed no matches to known genes. Quantitative RT-PCR analysis for selected eleven genes demonstrated that they are differentially expressed in roots of two radish cultivars according to the direction of subtraction. These results will contribute to a better understanding of the mechanisms and inheritance of radish root shape. |
| 出版者 |
|
|
出版者 |
JAPANESE SOCIETY OF BREEDING |
| 権利 |
|
|
権利情報 |
Copyright (c) 2010 by JAPANESE SOCIETY OF BREEDING |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1270/jsbbs.60.130 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
bs-v60n2p130-138.pdf (518.3 kB)
