{"created":"2023-05-15T12:04:41.054432+00:00","id":9247,"links":{},"metadata":{"_buckets":{"deposit":"e43f68c6-806e-445b-8316-42f6b3a42831"},"_deposit":{"created_by":3,"id":"9247","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"9247"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00009247","sets":["1504:1510"]},"author_link":["72461","72460","72464","72462","72463","72465"],"item_16_alternative_title_23":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Cold Response of Plant Microdomain-associated Proteins"}]},"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-12-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"162","bibliographicPageStart":"155","bibliographicVolumeNumber":"54","bibliographic_titles":[{"bibliographic_title":"低温生物工学会誌"}]}]},"item_16_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2010-12-06"}]},"item_16_description_12":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Arabidopsis thaliana increases freezing tolerance upon exposure to low, non-freezing temperatures, which is known as cold acclimation (CA). CA results in significant changes in the lipid and protein compositions in the plasma membrane and these changes affect the freezing tolerance of plants. Recently, domains in the plasma membrane (called as microdomains), which contain membrane specific proteins associated with membrane trafficking and signal transduction, are isolated as detergent-resistant plasma membrane fractions (DRM). To investigate the role of DRM-associated proteins in CA, we characterized cold-responsive DRM-associated proteins in Arabidopsis seedlings during CA. Two-dimensional differential gel electrophoresis and subsequent mass spectrometric analyses revealed that many proteins quantitatively changed during CA, among which P-type H^+-ATPase, aquaporin and endocytosis-related proteins increased and tubulin, actin and V-type H^+-ATPase subunits decreased in DRM. Gene expression analysis indicated that the protein accumulation levels in DRM during CA were not regulated at levels of transcription. These results suggest that (1) Arabidopsis plasma membrane microdomains play a role as a platform for membrane transport, membrane trafficking and cytoskeleton interaction and (2) the changes in these proteins are not correlated with the changes in gene expression levels during CA.","subitem_description_type":"Other"}]},"item_16_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"72463","nameIdentifierScheme":"WEKO"}],"names":[{"name":"MINAMI, Anzu"}]},{"nameIdentifiers":[{"nameIdentifier":"72464","nameIdentifierScheme":"WEKO"}],"names":[{"name":"FURUTO, Akari"}]},{"nameIdentifiers":[{"nameIdentifier":"72465","nameIdentifierScheme":"WEKO"}],"names":[{"name":"UEMURA, Matsuo"}]}]},"item_16_publisher_14":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"低温生物工学会"}]},"item_16_rights_18":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"低温生物工学会"},{"subitem_rights":"ご利用に当たっては「著作権法」に従うことをお願いいたします．"}]},"item_16_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1340-7902","subitem_source_identifier_type":"ISSN"}]},"item_16_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"岩手大学21世紀COEプログラム"},{"subitem_text_value":"岩手大学農学部附属寒冷バイオフロンティア研究センター"},{"subitem_text_value":"岩手大学21世紀COEプログラム, 岩手大学農学部附属寒冷バイオフロンティア研究センター"}]},"item_16_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"南, 杏鶴"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"古戸, あかり"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"上村, 松生"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-11-14"}],"displaytype":"detail","filename":"cc-v54n2p155-162.pdf","filesize":[{"value":"511.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"cc-v54n2p155-162.pdf","url":"https://iwate-u.repo.nii.ac.jp/record/9247/files/cc-v54n2p155-162.pdf"},"version_id":"8d7b2cd6-9e6e-4297-a0ea-3d6d4826e395"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Arabidopsis","subitem_subject_scheme":"Other"},{"subitem_subject":"低温馴化","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞膜","subitem_subject_scheme":"Other"},{"subitem_subject":"マイクロドメイン","subitem_subject_scheme":"Other"},{"subitem_subject":"タンパク質","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"植物細胞膜マイクロドメインタンパク質の低温応答性","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"植物細胞膜マイクロドメインタンパク質の低温応答性"}]},"item_type_id":"16","owner":"3","path":["1510"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-12-06"},"publish_date":"2010-12-06","publish_status":"0","recid":"9247","relation_version_is_last":true,"title":["植物細胞膜マイクロドメインタンパク質の低温応答性"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-16T11:09:17.338149+00:00"}