{"created":"2023-05-15T12:04:43.880999+00:00","id":9301,"links":{},"metadata":{"_buckets":{"deposit":"61507572-e3af-4fb8-ae3e-e3f48ce3811e"},"_deposit":{"created_by":3,"id":"9301","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"9301"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00009301","sets":["1504:1510"]},"author_link":["72666","72667","72665","72670","72669","72671","72672","72668"],"item_16_alternative_title_23":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Clinical evaluation of bovine adenosine deaminase activities in the colostrums"}]},"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-12-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"202","bibliographicPageStart":"197","bibliographicVolumeNumber":"1","bibliographic_titles":[{"bibliographic_title":"産業動物臨床医学雑誌"}]}]},"item_16_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2011-10-14"}]},"item_16_description_12":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"乳汁中のアデノシンデアミナーゼ（ADA）活性の臨床的意義を検討した．まず臨床的に健康な搾乳中のホルスタイン種成乳牛35頭の乳汁を初乳と常乳（泌乳初期，泌乳最盛期，泌乳中期，泌乳後期）に区分し，乳清中のADA活性値を測定した．常乳の乳清ADAは0.03±0.01IU/ℓと著しく低値だったが，初乳は11.9±2.9 IU/ℓ（平均±標準誤差，以下同）と，常乳に比べて高値を示した．次に，ホルスタイン種成乳牛11頭について，分娩前後の血清ADA活性値と末梢血リンパ球幼若化能を調べた．また初乳および分娩１週間後の乳清ADA活性値と初乳中リンパ球幼若化能を測定した．これらの結果は，乳汁CMT変法検査が陰性牛の非乳房炎牛（６頭）と陽性牛の潜在性乳房炎牛（５頭）に分けて検討した．\n分娩前後の血清ADA活性値は，潜在性乳房炎牛が非乳房炎牛に対して高値を示す傾向が認められ，初乳の乳清ADAでも非乳房炎牛が13.5±1.7 IU/ℓ，潜在性乳房炎牛が 23.0±5.1 IU/ℓで，潜在性乳房炎牛が非乳房炎牛に対して有意（p<0.05）な高値を示した．分娩前後の末梢血リンパ球幼若化能は，非乳房炎牛では分娩時に低下する傾向がみられたが，潜在性乳房炎牛ではそのような傾向は認められず，分娩時にも高い反応性を示した．初乳中リンパ球幼若化能は，いずれのマイトージェンで刺激した場合も，潜在性乳房炎牛が非乳房炎牛に対して著しい高値を示した．PHAによる刺激指数がきわめて高値を示\nした個体では初乳中ADA活性も高値を示した．以上のことから，潜在性乳房炎を伴う初乳中のADA活性は炎症反応により活性値が上昇するため，乳房炎診断を同時に行う必要性が考えられた．リンパ球幼若化能とADA活性値の明らかな相関は見出せなかったが，初乳中のADA活性値は乳汁中のリンパ球，とくにCD4＋T細胞機能を反映することが推測され，簡便なリンパ球系細胞の免疫能評価に応用できる可能性が示唆された．","subitem_description_type":"Other"},{"subitem_description":"To determine the significance of adenosine deaminase （ADA） in the bovine colostrum, first, colostrum and ordinary milk samples were collected from 35 healthy adult Holstein\ncows and analyzed for ADA activity. The mean ADA activity was 11.9±2.9 IU/ℓ in the colostrum,\nwhich was significantly higher than 0.03±0.01IU/ℓ in the ordinary milk. In the second experiment, 11 healthy adult Holstein cows were examined for serum ADA activity and by peripheral lymphocyte transformation（LT） test during the peripartum period. Whey ADA and LT were also examined for the colostrum. The results were compared between mastitis（M） and non-mastitis（NM） groups diagnosed by the modified California mastitis test. Serum ADA activities in the M group tended to be higher than those in the NM group. The whey ADA activity in the colostrums was 23.0±5.1 IU/ℓ in the M group and 13.5±1.7 IU/ℓ in the NM group with a significant difference between these groups. LT in the peripheral blood in the NM group tended to be lower at parturition, whereas that in the M group\nremained high even at parturition. LT in the colostrum in the M group was significantly higher than that in the NM group. Cows with high LT also had high whey ADA activities in the colostrums. These results suggest a possibility that ADA activity might be correlated with the number of CD4+ T cells in the colostrums. Because measurement of the whey ADA activity is very simple and rapid, colostrum ADA activity seems to be a potential indicator of immune status in the colostrums.","subitem_description_type":"Other"}]},"item_16_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"72669","nameIdentifierScheme":"WEKO"}],"names":[{"name":"SATO, Masahiko"}]},{"nameIdentifiers":[{"nameIdentifier":"72670","nameIdentifierScheme":"WEKO"}],"names":[{"name":"IMANISHI, Akiko"}]},{"nameIdentifiers":[{"nameIdentifier":"72671","nameIdentifierScheme":"WEKO"}],"names":[{"name":"OKADA, Keiji"}]},{"nameIdentifiers":[{"nameIdentifier":"72672","nameIdentifierScheme":"WEKO"}],"names":[{"name":"YASUDA, Jun"}]}]},"item_16_publisher_14":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本家畜臨床学会, 大動物臨床研究会"}]},"item_16_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1884-684X","subitem_source_identifier_type":"ISSN"}]},"item_16_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"岩手大学農学部, （現）東京大学大学院農学生命科学研究科"},{"subitem_text_value":"岩手大学農学部"},{"subitem_text_value":"岩手大学農学部"},{"subitem_text_value":"岩手大学農学部"}]},"item_16_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"佐藤, 雅彦"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"今西, 晶子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"岡田, 啓司"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"安田, 準"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-11-14"}],"displaytype":"detail","filename":"jjlac-v1n4p197-202.pdf","filesize":[{"value":"372.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"jjlac-v1n4p197-202.pdf","url":"https://iwate-u.repo.nii.ac.jp/record/9301/files/jjlac-v1n4p197-202.pdf"},"version_id":"aa515e50-b928-4db6-ae8d-c8a37fce89a4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"アデノシンディアミナーゼ（ADA）","subitem_subject_scheme":"Other"},{"subitem_subject":"初乳","subitem_subject_scheme":"Other"},{"subitem_subject":"ホルスタイン牛","subitem_subject_scheme":"Other"},{"subitem_subject":"リンパ球幼若化能試験","subitem_subject_scheme":"Other"},{"subitem_subject":"Adenosine Deanimase（ADA）","subitem_subject_scheme":"Other"},{"subitem_subject":"Colostrums","subitem_subject_scheme":"Other"},{"subitem_subject":"Holstein Cow","subitem_subject_scheme":"Other"},{"subitem_subject":"Lymphocyte Transformation Test（LTT）","subitem_subject_scheme":"Other"},{"subitem_subject":"Mastitis","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"乳牛の初乳中アデノシンディアミナーゼ活性の臨床的意義の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"乳牛の初乳中アデノシンディアミナーゼ活性の臨床的意義の検討"}]},"item_type_id":"16","owner":"3","path":["1510"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-10-14"},"publish_date":"2011-10-14","publish_status":"0","recid":"9301","relation_version_is_last":true,"title":["乳牛の初乳中アデノシンディアミナーゼ活性の臨床的意義の検討"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-16T11:06:58.960072+00:00"}