@article{oai:iwate-u.repo.nii.ac.jp:00009320,
 author = {高畑, 義人},
 issue = {4},
 journal = {育種學雜誌},
 month = {Dec},
 note = {普通ソバは異型花柱性の自家不和合性で,有用な遺伝子型を効率的に維持,増殖することが困難である.そこで組織培養による増殖法の確立を目的として,幼花序からの植物体再生について検討した.普通ソバおよび宿根ソバの幼花序をNAA,2,4-DおよびBAを含んだB5培地で培養した.普通ソバではNAA0〜2.O+BA O〜2.Omg/l添加で幼花序から直接不定芽が生じ,特にNAA O・2+BA1・Omg/l添加区で最高の分化率を示し,岩手在来秋ソバで40.O%,階上早生で47.6%の不定芽形成がみられた(Table1,Fi9・1A).宿根ソバはNAA1.O+BA1.0mg/l区でのみ不定芽が生じ,その頻度も低かった(Table2).一方,両種ともNAA2.0mg/l以上あるいは2,4-D1.Omg/l以上の添加により高いカルス形成率を示した.これらのカルスをホルモン無添加あるいはNAA0.2+BA 1.0mg/l添加の再分化培地に継代したところ,普通ソバでは17.5〜28.6%の頻度で芽を分化したが,宿根ソバではまったく分化しなかった(Tab1e3,Fig.1B).幼花序から直接分化した場合も,カルス経由の場合も,芽をIBA1.Omg/l添加のMS培地に移手直し発根させた(Fig.1C).幼花序から直接再生した植物体のうち,普通ソバ25個体,宿根ソバ1個体について染色体数を調査したところ,すべて2n=16の2倍体であった., Maintenance of valuable genotypes of common buckwheat is difiicult owing to heterostylic self-incompatibility. To establish vegetative propagation system, a procedure for plant regeneration from immature inflorescence culture was developed. Immature inflorescences of common buckwheat and perennial buckwheat were cultured on B5 media supplemented with NAA, 2,4-D and BA at various concentrations. Direct shoot production from the inflorescence of common buckwheat was promoted by the addition of 0-2.0 mg/l NAA+0-2.0mg/l BA (optimum. 0.2 mg/l NAA+1. 0 mg/l BA). In perennial buckwheat, direct shoot was induced only on the medium containing 1.0mg/l NAA and l.0mg/l BA with lower frequency. than common buckwheat. In the presence of more than 1.0mg/l 2,4-D or more than 2.0mg/l NAA, callus was well formed in both species. Shoots were induced from common. buckwheat callus on B5 medium containing 0.2 mg/l NAA and 1.0mg/l BA and lacking plant growth regulators, but not from perennial buckwheat. The shoots obtained by the two different pathways developed a root system on MS medium containing 1.0 mg/l IBA. Chromosomal analysis of the directly induced regenerants showed that they had diploid chromosome numbers (2 n=16).},
 pages = {409--413},
 title = {Plant Regeneration from Cultured Immature Inflorescence of Common Buckwheat (Fagopyrum esculentum MOENCH) and Perennial Buckwheat (F.cymosum MEISN.)},
 volume = {38},
 year = {1988}
}