{"created":"2023-05-15T12:04:44.767073+00:00","id":9320,"links":{},"metadata":{"_buckets":{"deposit":"b26035ef-b801-4aa9-aa04-67d9674aafa9"},"_deposit":{"created_by":3,"id":"9320","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"9320"},"status":"published"},"_oai":{"id":"oai:iwate-u.repo.nii.ac.jp:00009320","sets":["1504:1510"]},"author_link":["72813","72812"],"item_16_alternative_title_23":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"普通ソバ(Fagopyrum esculentum MOENCH)および宿根ソバ(F.cymosum MEISN.)の切花序からの植物体再生"}]},"item_16_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1988-12-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"413","bibliographicPageStart":"409","bibliographicVolumeNumber":"38","bibliographic_titles":[{"bibliographic_title":"育種學雜誌"}]}]},"item_16_date_6":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2012-01-13"}]},"item_16_description_12":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"普通ソバは異型花柱性の自家不和合性で,有用な遺伝子型を効率的に維持,増殖することが困難である.そこで組織培養による増殖法の確立を目的として,幼花序からの植物体再生について検討した.普通ソバおよび宿根ソバの幼花序をNAA,2,4-DおよびBAを含んだB5培地で培養した.普通ソバではNAA0〜2.O+BA O〜2.Omg/l添加で幼花序から直接不定芽が生じ,特にNAA O・2+BA1・Omg/l添加区で最高の分化率を示し,岩手在来秋ソバで40.O%,階上早生で47.6%の不定芽形成がみられた(Table1,Fi9・1A).宿根ソバはNAA1.O+BA1.0mg/l区でのみ不定芽が生じ,その頻度も低かった(Table2).一方,両種ともNAA2.0mg/l以上あるいは2,4-D1.Omg/l以上の添加により高いカルス形成率を示した.これらのカルスをホルモン無添加あるいはNAA0.2+BA 1.0mg/l添加の再分化培地に継代したところ,普通ソバでは17.5〜28.6%の頻度で芽を分化したが,宿根ソバではまったく分化しなかった(Tab1e3,Fig.1B).幼花序から直接分化した場合も,カルス経由の場合も,芽をIBA1.Omg/l添加のMS培地に移手直し発根させた(Fig.1C).幼花序から直接再生した植物体のうち,普通ソバ25個体,宿根ソバ1個体について染色体数を調査したところ,すべて2n=16の2倍体であった.","subitem_description_type":"Other"},{"subitem_description":"Maintenance of valuable genotypes of common buckwheat is difiicult owing to heterostylic self-incompatibility. To establish vegetative propagation system, a procedure for plant regeneration from immature inflorescence culture was developed. Immature inflorescences of common buckwheat and perennial buckwheat were cultured on B5 media supplemented with NAA, 2,4-D and BA at various concentrations. Direct shoot production from the inflorescence of common buckwheat was promoted by the addition of 0-2.0 mg/l NAA+0-2.0mg/l BA (optimum. 0.2 mg/l NAA+1. 0 mg/l BA). In perennial buckwheat, direct shoot was induced only on the medium containing 1.0mg/l NAA and l.0mg/l BA with lower frequency. than common buckwheat. In the presence of more than 1.0mg/l 2,4-D or more than 2.0mg/l NAA, callus was well formed in both species. Shoots were induced from common. buckwheat callus on B5 medium containing 0.2 mg/l NAA and 1.0mg/l BA and lacking plant growth regulators, but not from perennial buckwheat. The shoots obtained by the two different pathways developed a root system on MS medium containing 1.0 mg/l IBA. Chromosomal analysis of the directly induced regenerants showed that they had diploid chromosome numbers (2 n=16).","subitem_description_type":"Other"}]},"item_16_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"72813","nameIdentifierScheme":"WEKO"}],"names":[{"name":"TAKAHATA, Yoshihito"}]}]},"item_16_publisher_14":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本育種学会"}]},"item_16_relation_16":{"attribute_name":"異版である","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://ci.nii.ac.jp/naid/110001815735","subitem_relation_type_select":"URI"}}]},"item_16_rights_18":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"日本育種学会"},{"subitem_rights":"本文データは学協会の許諾に基づきCiNiiから複製したものである"}]},"item_16_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0536-3683","subitem_source_identifier_type":"ISSN"}]},"item_16_text_4":{"attribute_name":"著者(機関)","attribute_value_mlt":[{"subitem_text_value":"岩手大学農学部"}]},"item_16_version_type_27":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"高畑, 義人"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-11-14"}],"displaytype":"detail","filename":"jjb-v38n4p409-413.pdf","filesize":[{"value":"670.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"jjb-v38n4p409-413.pdf","url":"https://iwate-u.repo.nii.ac.jp/record/9320/files/jjb-v38n4p409-413.pdf"},"version_id":"558676c9-9de8-4329-97b8-9b5fbf518bc8"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Fagopyrum esculentum","subitem_subject_scheme":"Other"},{"subitem_subject":"F.cymosum","subitem_subject_scheme":"Other"},{"subitem_subject":"inflorescence","subitem_subject_scheme":"Other"},{"subitem_subject":"plant regeneration","subitem_subject_scheme":"Other"},{"subitem_subject":"tissue culture","subitem_subject_scheme":"Other"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Plant Regeneration from Cultured Immature Inflorescence of Common Buckwheat (Fagopyrum esculentum MOENCH) and Perennial Buckwheat (F.cymosum MEISN.)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Plant Regeneration from Cultured Immature Inflorescence of Common Buckwheat (Fagopyrum esculentum MOENCH) and Perennial Buckwheat (F.cymosum MEISN.)"}]},"item_type_id":"16","owner":"3","path":["1510"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-01-13"},"publish_date":"2012-01-13","publish_status":"0","recid":"9320","relation_version_is_last":true,"title":["Plant Regeneration from Cultured Immature Inflorescence of Common Buckwheat (Fagopyrum esculentum MOENCH) and Perennial Buckwheat (F.cymosum MEISN.)"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-16T11:06:13.097579+00:00"}