| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2012-01-16 |
| タイトル |
|
|
タイトル |
Generation of expressed sequence tags, development of microsatellite and single nucleotide polymorphism markers in Primula sieboldii E. Morren (Primulaceae) for analysis of genetic diversity in natural and horticultural populations |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
genetic resource |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
horticulture |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
heterostyly |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
gene ontology |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
SNP |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
EST-SSR |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
SNaPshot |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Ueno, Saneyoshi
Yoshida, Yasuko
Taguchi, Yuriko
Ujino-Ihara, Tokuko
Kitamoto, Naoko
Honjo, Masanori
Ohsawa, Ryo
Washitani, Izumi
Tsumura, Yoshihiko
|
| 著者(機関) |
|
|
値 |
Tree Genetics Laboratory, Department of Forest Genetics, Forestry and Forest Products Research Institute |
| 著者(機関) |
|
|
値 |
Graduate School of Life and Environmental Sciences, University of Tsukuba |
| 著者(機関) |
|
|
値 |
Tree Genetics Laboratory, Department of Forest Genetics, Forestry and Forest Products Research Institute |
| 著者(機関) |
|
|
値 |
Tree Genetics Laboratory, Department of Forest Genetics, Forestry and Forest Products Research Institute |
| 著者(機関) |
|
|
値 |
Faculty of Agriculture, Iwate University |
| 著者(機関) |
|
|
値 |
National Agricultural Research Center for Tohoku Region |
| 著者(機関) |
|
|
値 |
Graduate School of Life and Environmental Sciences, University of Tsukuba |
| 著者(機関) |
|
|
値 |
Graduate School of Agricultural and Life Science, The University of Tokyo |
| 著者(機関) |
|
|
値 |
Tree Genetics Laboratory, Department of Forest Genetics, Forestry and Forest Products Research Institute |
| 登録日 |
|
|
日付 |
2012-01-16 |
| 書誌情報 |
Breeding Science
巻 61,
号 3,
p. 234-243,
発行日 2011-01-01
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1344-7610 |
| Abstract |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Primula sieboldii is a self-incompatible ornamental plant that has been cultivated for more than 300 years in Japan. In order to increase the available genomic resources for this species, 5651 expressed sequence tags (ESTs) were generated from seedling and winter bud cDNA libraries. After clustering and assembling ESTs, 2960 unigene elements that contain an homologous sequence to an S locus-linked gene of P. vulgaris were identified. In total, 127 simple sequence repeats (SSRs) were found. The most frequent di- and tri-SSR motifs were GA (78.6%) and ATT (12.9%), respectively. Twenty SSR and 40 single nucleotide polymorphism (SNP) markers were developed. These markers were evaluated for polymorphisms using 24 individuals (Y) from a population in Yatsugatake and 14 individuals (PS) from across the distributional range of P. sieboldii in Japan. The average level of expected heterozygosity within Y and PS was 0.39 and 0.48, respectively for EST-SSR markers and 0.26 and 0.29, respectively for SNP markers. The level of polymorphism for EST-SSR markers was lower than that for genomic SSR markers developed in a previous study. We also surveyed the genetic diversity of 56 cultivars, which had lower allelic richness than the Y and PS populations. |
| 出版者 |
|
|
出版者 |
JAPANESE SOCIETY OF BREEDING |
| 権利 |
|
|
権利情報 |
Copyright (c) 2011 by JAPANESE SOCIETY OF BREEDING |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1270/jsbbs.61.234 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
bs-v61n3p234-243.pdf (569.5 kB)
